Effect of hydrogen peroxide on the fluorescence and the C550 absorption change of spinach chloroplast fragments
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概要
- 論文の詳細を見る
Effects of H_2O_2 on the transient phase of fluorescence and the light-induced absorption change of C550 in the presence of ferricyanide were studied in spinach chloroplast fragments at room temperature. In the presence of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), the parameter of the variable fluorescence, work integral, was increased by the addition of H_2O_2 and the rate of its recovery in the dark was decreased. The steady-state fluorescence yield was decreased by H_2O_2. Essentially the same results were obtained in the absence of ferricyanide. In the presence of DCMU, H_2O_2 decreased the steady-state absorption change of C550 and inhibited its reoxidation in the dark. The same effects were observed when H_2O_2 was added to chloroplast fragments in the presence of DCMU and carbonyl cyanide m-chlorophenylhydrazone. From these data we concluded that the fluorescence quencher Q and C550 are not identical.
- 日本植物生理学会の論文
著者
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INOUE Hiroshi
Department of Internal Medicine, University of Toyama
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Nishimura Mitsuo
Department of Biology, Faculty of Science, Kyushu University
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Nishimura Mitsuo
Departmcnt Of Biology Faculty Of Science Kyushu University
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Takahama Umeo
Departmcnt of Biology, Faculty of Science, Kyushu University
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Takahama Umeo
Departmcnt Of Biology Faculty Of Science Kyushu University
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Inoue Hiroshi
Department Of Electrical And Electronic Engineering Akita University
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Inoue Hiroshi
Departmcnt Of Biology Faculty Of Science Kyushu University:(present)department Of Biology Kyushu Den
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Inoue Hiroshi
Deparment of Neurosurgery, Gunma University School of Medicine and Gamma Unit Center, Hidaka Hospital
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