Isolation and identification of Rothia mucilaginosa from persistent apical periodontitis lesions

概要

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We report two bacterial strains isolated from individual persistent apical periodontitis lesions where the presence of bacteria was noted in root canals after numerous treatments. Following sampling from the root canals, a representative single colony was randomly selected from each case for further study. These strains were identified as Rothia mucilaginosa by 16S ribosomal RNA gene sequencing. Scanning electron microscopy observation revealed that these isolates possessed unique phenotypic characteristics by exhibiting dense meshwork-like structures on their cell surfaces, which had a similar appearance of structure typically possessed by a number of biofilm-forming bacterial species. These results suggest that Rothia mucilaginosa isolated from persistent apical periodontitis lesions, which possess dense meshwork-like structures on their cell surface, might form biofilms to survive in host environments.