92(P-18) 昆虫寄生糸状菌をはじめとする微生物の自然免疫制御物質(ポスター発表の部)

概要

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Innate immunity is the first line of defense against infectious microorganisms. The basic mechanisms of pathogen recognition and response activation are evolutionarily conserved. In mammals, the innate immune response is required for the activation of acquired immunity. Therefore, innate immunity is a pharmaceutical target for the development of immune regulator. For pharmaceutical screening, we established an in vitro culture based on the innate immune response of Drosophila, which is equivalent to that of mammals. The expression of a reporter gene, Dpt-lac Z, reflected the LPS-dependent activation of innate immunity. In combination with the loss-of-function and gain-of-function mutation in the Drosophila immune response, the culture system has an advantage over conventional cell-line culture in identifying the target molecules that the chemicals affected. We screened the effect of ca. 400 extracts of microorganism including entomopathogenic fungi on innate immunity. TP-1 (1), ceramide 2 and cephalosporolide B (7) were isolated as specific inhibitor of the innate immune system. For further biological study, TP-1 (1) was synthesized from commercially available 3-cyclopentene-1-carboxylic acid.
2004-10-01