ヒト歯肉培養線維芽細胞によるコラーゲン線維の貪食と細胞内消化について

概要

論文の詳細を見る
The process of collagen phagocytosis and degradation by human gingival fibroblasts were studied in vitro by means of electron microscopy and histochemistry. In an attempt to demonstrate the phagocytic nature of intracytoplasmic vacuoles, monolayer cultures of human gingival fibroblasts were incubated with collagen fibers for 3 to 6 days and were observed with an electron microcope. Some samples were examined at the ultrastructural level for acid phosphatase activity. The following results were obtained: 1) Electron microscopy demonstrated collagen fibrils in numerous intracytoplasmic vacuoles in the cells. 2) The vacuoles in fibroblasts cultured for 3 days contained an electron-lucent matrix, whereas those in cells cultured for 6 days frequently contained an electron-dense matrix. 3) Some of these structures containing collagen fibrils showed a positive acid phosphatase reaction. These results strongly suggest that the intracytoplasmic vacuoles are phagosomes and the phagocytosed collagen fibrils are digested by lysosomal enzymes.
特定非営利活動法人日本歯周病学会の論文
1982-09-28