ノダムラウイルスに関する研究 : 血清学的診断法の検討

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Nodamura virus is an unusual arthropod-borne orphan virus isolated from Japanese mosquitoes, because it was capable of multiplication in arthropods, and of being multiplied biologically by mosquitoes, but unlike other arbovirus, its infectivity is not inactivated by diethylether and chloroform. From such characteristics as being arthropod-borne and developing paralysis of hind limb in the susceptible suckling mice, this virus was once suspected to be a causal agent of Akabane disease : epizootic congenital arthrogryposis-hydranencephaly syndrome of cattle breaking out during 1972-1974 in Japan. When Akabane virus was established as a causal agent of the disease, Nodamura virus still remains as an orphan virus. However, it is important to pay attention on such a interesting arthropod-borne virus if we are reminded of Akabane disease story. This paper deals with some attempts to show the methods for serological diagnosis or survey of Nodamura virus concerning the possible prevalence of the disease caused by Nodamura virus. Complement fixation test and gel precipitation test with variable antigen preparations made directly from the infected mouse limb emulsion were tested under differnt conditions and the following results were obtained. 1) Optimum condition for CFT were ascertained to be tested by small amount method, using 4 units of antigen, 2 units of complement and hemolysin system consisting of 3 units of hemolysin and equal amount of 2.5% sheep red cell. Optimum sensitization of the reacting system was to be kept at 4℃ for 18 hours. 2) Indirect complement fixation test was available for bovine serum, under the condition of 2 units of antigen and 2 units of indicating mouse serum. 3) Centrifuged supernate of the tissue and the Daiflon treated emulsion supernate were supposed to be the most sensitive antigens for both CFT and GPT. Especially partially purified Daiflon treated antigen would be looked upon as an adequate antigen for practical survey of animals. 4) The complement fixing antigen of Nodamura virus consisted of two fractions such as heat stable and heat labile ones which were to be devided by being heated at 70℃ for 30 minutes. 5) As for developing of antibodies, CF-antibody was detectable in the second week and later after inoculation, while GP-antibody was to be detected in the first week, and none of CF-antibody activity was noted to be in IgM fraction. 6) None of the positive serum was demonstated from the 911 serum samples taken out of variable animals in southern Kyushu area.
鹿児島大学の論文
1978-03-19

ノダムラウイルスに関する研究 : 血清学的診断法の検討

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