Development and Characterization of a Monoclonal Antibody to Phytosiderophores
スポンサーリンク
概要
- 論文の詳細を見る
Mugineic acid-family phytosiderophores (MAs) are low molecular weight chelators that are secreted by graminaceous plants, form complexes with soil Fe(III) and are essential for plant growth. Methods to detect MAs which include HPLC and radio-immunoassay with polyclonal antibody require sophisticated equipment or radio-labelled MAs which are difficult to synthesize. Our objective was to develop a detection and quantitation system for MAs based on monoclonal antibody specificity and technology. A monoclonal antibody was produced which reacts with nicotianamine (NA), deoxymugineic acid (DMA), mugineic acid (MA) and epi-hydroxymugineic acid (epi-HMA) in a competitive ELISA. Azetidine-2-carboxylic acid (A-2-C) was not reactive while N-(3-amino-3-carboxypropyl) azetidine-2-carboxylic acid (A-2-C dimer) was partially reactive. The range of detection using the competitive ELISA is from 2×10^<16> to 2×10^<-7> M MAs. Besides detection and quantification of MAs, the potential uses for the monoclonal antibody are numerous and include affinity chromatography and immunocyiochemistry.
- 日本植物生理学会の論文
著者
-
MORI Satoshi
Department of Applied Biological Chemistry, Division of Agriculture and Agricultural Life Sciences,
-
Mori S
Dept. Of Appl. Biol. Chem. Univ. Tokyo
-
Mori Satoshi
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
-
Fushiya Shinji
Pharmaceutical Institute, Tohoku University
-
Mihashi Shuichi
Department of Agricultural Chemistry, The University of Tokyo
-
Sikora Lawrence
Soil-Microbial Systems Laboratory, USDA -ARS, Beltsville Agricultural Research Center
-
Buyer Jeffrey
Soil-Microbial Systems Laboratory, USDA -ARS, Beltsville Agricultural Research Center
-
FUSHIYA Shinji
Department of Kampo Pharmaceutical Sciences, Nihon Pharmaceutical University
-
Mori Satoshi
Lab Plant. Mol. Physiol. Tokyo Univ. Fac. Pharmaceutical. Science. Nagoya City Univ.
-
Mori S
Department Of Applied Biological Chemistry The University Of Tokyo
-
Buyer Jeffrey
Soil-microbial Systems Laboratory Usda -ars Beltsville Agricultural Research Center
-
Fushiya S
Department Of Kampo Pharmaceutical Sciences Nihon Pharmaceutical University
-
Sikora Lawrence
Soil-microbial Systems Laboratory Usda -ars Beltsville Agricultural Research Center
関連論文
- Comparison of the functions of the barley nicotianamine synthase gene HvNAS1 and rice nicotianamine synthase gene OsNAS1 promoters in response to iron deficiency in transgenic tobacco(Plant Nutrition)
- Transgenic rice lines that include barley genes have increased tolerance to low iron availability in a calcareous paddy soil(Plant Nutrition)
- Detection and Characterization of Protein Kinases in Rice Phloem Sap : ENVIRONMENTAL AND STRESS RESPONSES : PROTEINS, ENZYMES AND METABOLISM
- Ultrastructural Localization of the Basic 7S Globulin in Soybean(Glycine max) Cotyledons : GROWTH AND DEVELTOPMENT : STRUCTURE AND FUNCTION OF CELLS
- Effects of unipedal standing balance exercise on the prevention of falls and hip fracture among clinically defined high-risk elderly individuals : a randomized controlled trial
- Report on the Japanese Orthopaedic Association's 3-year project observing hip fractures at fixed-point hospitals
- Synthesis of nicotianamine and deoxymugineic acid is regulated by OsIRO2 in Zn excess rice plants(Plant Nutrition)
- How are metal nutrients transported to the embryo in rice plant?
- Overcoming Fe deficiency in guava (Psidium guajava L.) by co-situs application of controlled release fertilizers(Fertilizer and Soil Amendments)
- ^Mn translocation in barley monitored using a positron-emitting tracer imaging system(Plant Nutrition)
- 9-22 Characterization of Glutathione transporter in rice
- Iron deficiency enhances cadmium uptake and translocation mediated by the Fe^ transporters OsIRT1 and OsIRT2 in rice(Plant Nutrition)
- Metabolic Engineering of Saccharomyces cerevisiae Producing Nicotianamine : Potential for Industrial Biosynthesis of a Novel Antihypertensive Substrate
- Construction of Artificial Promoters Highly Responsive to Iron Deficiency
- Directed Evolution of Yeast Ferric Reductase to Produce Plants with Tolerance to Iron Deficiency in Alkaline Soils
- A Rice FRD3-Like (OsFRDL1) Gene Is Expressed in the Cells Involved in Long-Distance Transport
- Diurnal Changes in the Expression of Genes That Participate in Phytosiderophore Synthesis in Rice
- H_2^O Translocation in Rice Was Enhanced by 10 μM 5-Aminolevulinic Acid as Monitored by Positron Emitting Tracer Imaging System (PETIS)
- Analysis of Upstream Region of Nicotianamine Synthase Gene from Arabidopsis thaliana : Presence of Putative ERE-like Sequence(Biochemistry & Molecular Biology)
- The Expression of a Barley HvNAS1 Nicotianamine Synthase Gene Promoter-gus Fusion Gene in Transgenic Tobacco is Induced by Fe-deficiency in Roots(Biochemistry & Molecular Biology)
- Analysis of Transgenic Rice Containing Barley Nicotianamine Synthase Gene
- ID17,A NEW IRON-REGULATED ABC TRANSPORTER FROM BARLEY ROOTS, LOCALIZED TO THE TONOPLAST
- ANALYSIS OF UPSTREAM REGION OF NICOTIANAMINE SYNTHASE GENES FROM Arabidopsis thaliana (II)
- Protein Phosphorylation in the Sieve Tubes of Rice Plants
- Histiocytoid breast carcinoma : Solid variant of invasive lobular carcinoma with decreased expression of both E-cadherin and CD44 epithelial variant
- Post-Translational Modification of βH-Crystallin of Bovine Lens with Aging (Medicina Chemistry)
- Effects of Anion Channel Blockers on Xylem Nitrate Transport in Barley Seedlings
- Absorption and Translocation of ^Fe, ^Mn and ^Zn Visualized by PETIS in Barley and Rice
- Visualization of ^O-Water Flow in Tomato and Rice in the Light and Dark Using a Positron-Emitting Tracer Imaging System (PETIS)
- Analysis of ^NH_4^+ uptake, translocation and retranslocation in rice by PETIS (Positron Emitting Tracer Imaging System)
- Absorption and Translocation of ^Mn Visualized by PETIS in Barley and Tomato
- Translocation of ^C-Methionine Visualized by Positron Emitting Tracer Imaging System (PETIS) in Barley
- Plasma Opening Switch Using Plasmas Produced by a Pulsed CO_2 Laser
- Importance of contact between rice roots and co-situs applied fertilizer granules on iron absorption by paddy rice in a calcareous paddy soil(Fertilizers and Soil Amendments)
- Establishment of a Cell-Free System for the Biosynthesis of Nicotianamine
- Light Regulation of Hypocotyl Elongation and Greening in Transgenic Tobacco Seedlings That Over-Express Rice Phytochrome A
- CHARACTERIZATION OF TWO PROTEINS INDUCED BY IRON DEFICIENCY IN BARLEY ROOTS
- STUDY ON THE BIOSYNTHETIC PATHWAY OF MUGINEIC ACID IN Fe-DEFICIENT BARLEY ROOTS V : PURIFICATION OF NICOTIANAMINE AMINOTRANSFERASE
- Distinction of water-soluble constituents between some Paecilomyces (= Isaria) and Cordyceps fungi by capillary electrophoresis
- Screening of Mongolian Plants for Influence on Amylase Activity in Mouse Plasma and Gastrointestinal Tube
- Changes of bone mineral density of osteoporotic patients treated with bisphosphonates
- Determination of the N-Terminal Amino Acid Sequence of Mungbean Leghemoglobin
- Induction of Adenine Phosphoribosyltransferase (APRT) in Iron-deficient Barley Roots
- Biosynthetic Pathway of Phytosiderophores in Iron-Deficient Graminaceous Plants : Development of an Assay System for the Detection of Nicotianamine Aminotransferase Activity
- Development and Characterization of a Monoclonal Antibody to Phytosiderophores
- Cross-Reactivities of Anti-Nicotianamine Antibody with Mugineic Acid and Related Compounds
- Eel calcitonin (elcatonin) suppressed callus remodeling but did not interfere with fracture healing in the femoral fracture model of cynomolgus monkeys
- A case of femoral nerve palsy caused by iliopectineal bursitis associated with rheumatoid arthritis
- Relationships between Changes in Oxygenation during Exercise and Recovery in Trained Athletes
- Changes of total body bone mineral density after parathyroidectomy in hemodialyzed patients
- Effects of single and concurrent intermittent administration of human parathyroid hormone and bisphosphonate cimadronate on bone mineral densities of femur in ovariectomized rats
- Spatial Current Distribution Inside the Switch Region of a Plasma Opening Switch with Coaxial Configuration
- Effect of c-fos overexpression on development and proliferation of peritoneal B cells
- The Liquid Culture Filtrates of Entomogenous fungus Paecilomyces tenuipes and Its Glycoprotein Constituent Protects against Anemia in Mice Treated with 5-Fluorouracil(Pharmacognosy)
- Water (H_2 ^15O) Flow in Rice is Regulated by the Concentration of Nutrients as Monitored by Positron Multi-Probe System (PMPS)
- Suppression of NO Production in Activated Macrophages in Vitro and ex Vivo by Neoandrographolide Isolated from Andrographis paniculata
- The Culture Fluid of Isaria japonica YASUDA Augments Anti-Sheep Red Blood Cell Antibody Response in Mice
- IN-VIVO IDENTIFICATION OF MUGINEIC ACID SYNTHASE GENE USING TRANSGENIC RICE
- Induction of the IDI1 Gene in Fe-Deficient Barley Roots: A Gene Encoding a Putative Enzyme That Catalyses the Methionine Salvage Pathway for Phytosiderophore Production
- ISOLATION OF A cDNA FOR AN FE-DEFICIENCY INDUCIBLE LIPOXYGENASE ISOFORM FROM BARLEY
- ANALYSIS OF UPSTREAM REGION OF NICOTIANAMINE SYNTHASE GENES FROM Arabidopsis thaliana
- TRANSGENIC RICE AND TOBACCO CONTAINING CaMV35S-NICOTIANAMINE SYNTHASE GENE (HVNAS1)
- THE TOBACCO OVEREXPRESSED WITH NAAT-A GENE SHOWED INTERCOSTAL CHLOROSIS
- Cloning of Nicotianamine Synthase Genes from Arabidopsis thaliana
- Time Course Study of Aluminum-Induced Callose Formation in Barley Roots as observed by Digital Microscopy and Low-Vacuum Scanning Electron Microscopy
- Presence of Nicotianamine Synthase Isozymes and Their Homologues in the Root of Graminaceous Plants
- Conforcal Images of Adenine Phosphoribosyltransferase (APRT) Fused to sGFP in Plant Cells
- THE FUNCTIONAL ANALYSIS OF BARLEY SFD1 GENE, A SUPPRESSOR OF YEAST COPPER UPTAKE MUTANT ctr 1
- CLONING AND CHARACTERIZATION OF NICOTIANAMINE SNTHASE GENES FROM Arabidopsis thaliana
- CLONING OF NICOTIANAMINE SYNTHASE GENES (nas) FROM GRAMINACEOUS PLANTS
- DEVELOPMENT OF THE TRANSGENIC RICE OVEREXPRESSING NICOTIANAMINE AMINOTRANSFERASE
- Methionine as a Dominant Precursor of Phytosiderophores in Graminaceae Plants
- Difference in Al Sensitivity between Seminal and Crown Roots of Rice Nursery Seedlings (Plant Nutrition)
- Extraordinary High Aluminium Tolerance of the Acidophilic Thermophilic Alga, Cyanidium caldarium
- Co-situs Application of Controlled-Release Fertilizers to Alleviate Iron Chlorosis of Paddy Rice Grown in Calcareous Soil
- ライ麦染色体添加系統を用いた2′-デオキシムギネ酸合成酵素および3ハイドロキシムギネ酸合成酵素の遺伝子座乗染色体ライムギ5Rの決定〔英文〕
- Preadministration of Incadronate Disodium Prevent Bone Loss in Rat's Proximal Tibiae Induced by Ovariectomy at 8 weeks after Administration
- Long-Term Effects of Incadronate Disodium(YM175) on Fracture Healing of Rat's Femur
- Effect of growth Howmone on Growth Failure and Bone Loss Induced by Supraphysiologic Dose of Glucocorticoid in Rats
- MICROBODIES CONTAINING CRYSTALLOID INCLUSIONS IN RICE ROOT CELLS
- Ultrastructre of the Thylakoid Membrane in Tomato Leaf Chloroplast Revealed by Liquid Helium Rapid-Freezing and Substitution-Fixation Method
- Current trends in bone grafting and the issue of banked bone allografts based on the fourth nationwide survey of bone grafting status from 2000 to 2004
- ^Fe Translocation in Barley as Monitored by a Positron-Emitting Tracer Imaging System (PETIS) : Evidence for the Direct Translocation of Fe from Roots to Young Leaves via Phloem
- Analysis of interobserver reliability for radiographic staging of coxarthrosis and indexes of acetabular dysplasia : a preliminary study
- Ultrasonographic Evaluation of the Function of Respiratory Muscles during Breathing Exercises
- Multiracial/Multiethnic Identity : A Study of "Mixed" Yonsei in Hawai'i
- STIMULATION OF RELEASE OF REDUCING SUGARS FROM THE ENDOSPERMS OF RICE SEEDS BY HELMINTHOSPOROL
- Highly Sensitive Quantitative Analysis of Nicotianamine Using LC/ESI-TOF-MS with an Internal Standard
- Multiinstitutional epidemiological study regarding osteoarthritis of the hip in Japan
- Nationwide one-decade survey of hip fractures in Japan
- A High Performance Liquid Chromatography-Radio-Organic Acid Analyzer for Determining the Specific Activities of Radioactive Organic Acids
- Identification of a Jasmonic Acid-Responsive Region in the Promoter of the Rice 12-Oxophytodienoic Acid Reductase 1 Gene OsOPR1
- Excess Copper Induces A Cytosolic Cu,Zn-Superoxide Dismutase in Soybean Root
- Radical Scavenging Activities of Condensed Culture Medium of Isaria japonica Yasuda and Hot Water Extract of Fomes fomentarius (L.:Fr) Kicrx
- Survey of the Influence of Mongolian Plants on Lipase Activity in Mouse Plasma and Gastrointestinal Tube
- 資料 Effects of Mongolian Plants on Nitric Oxide Production in Activated Macrophages
- Osteoarthritis hip joints in Japan : involvement of acetabular dysplasia
- Structure of mugineic acid, a new amino acid possessing an iron-chelating activity from roots washings of water-cultured Hordeum vulgare L.
- DETECTION OF ORGANIC ACIDS IN SOYBEAN NODULES BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
- Justification criteria for vertebral fractures : year 2012 revision