Analysis of Transgenic Rice Containing Barley Nicotianamine Synthase Gene
スポンサーリンク
概要
- 論文の詳細を見る
Nicotianamine (NA) is an intermediate in the biosynthetic pathway of mugineic acid-family phytosiderophores (MAs), which are essential components of the iron acquisition mechanism of graminaceous plants. NA is also considered to be an essential chelator of metal cations in dicots. Thus, NA and nicotian-amine synthase (NAS, EC 2.5.1.43) play important roles in Fe metabolism and homeostasis in higher plants. To evaluate the contribution of NAS to MAs biosynthesis, we introduced the barley hvnas1 gene under the control of the CaMV35S promoter into rice. Transgenic rice containing a barley HvNAS1 genomic DNA fragment was also analyzed. Although significant expression of hvnas1 driven by the CaMV35S promoter was confirmed by Northern and Western analyses, total NAS activity, endogenous NA, and 2'-deoxymugineic acid (DMA) secretion were not higher under Fe-deficient conditions compared to vector control plants. However, under Fe-sufficient conditions, the amounts of endogenous NA and secreted DMA increased slightly. No accumulation of NA due to an altered balance of nicotianamine aminotransferase (NAAT) and NAS activities was observed. These results indicate that the NAS activity may be strictly regulated.
- 社団法人日本土壌肥料学会の論文
著者
-
Nishizawa Naoko
Laboratory Of Plant Biotechnology Graduate School Of Agricultural And Life Sciences The University O
-
KAWASAKI Shinji
National Institute of Agrobiological Sciences
-
Mori S
Laboratory Of Plant Biotechnology Graduate School Of Agricultural And Life Sciences The University O
-
Kawasaki S
Kyokugen Research Center For Materials Science At Extreme Conditions Osaka University
-
MORI Satoshi
Laboratory of Plant Biotechnology, Graduate School of Agricultural and Life Sciences, The University
-
HIGUCHI Kyoko
Laboratory of Plant Production Chemistry, Department of Applied Biology and Chemistry, Tokyo Univers
-
Kim Suyeon
Department of Global Agricultural Sciences, The University of Tokyo
-
NAKANISHI Hiromi
Laboratory of Plant Molecular Physiology, Department of Applied Biological Chemistry, The University
-
Takahashi Michiko
Laboratory of Plant Molecular Physiology, Department of Applied Biological Chemistry, The University
-
Kawasaki Shinji
Japex Geosciences Institute Tokyo Japan
-
Nishizawa Naoko-kishi
Lab Plant. Mol. Physiol. Tokyo Univ. Fac. Pharmaceutical. Science. Nagoya City Univ.
-
Mori Satoshi
Laboratory Of Piant Molecular Physiology Department Of Applied Biological Chemistry The University O
-
Kawasaki Shinji
National Institute Of Agrobiological Resources Tsukuba
-
Takahashi Michiko
Department Of Global Agricultural Sciences Graduate School Of Agricultural And Life Science The Univ
-
Mori Satoshi
Lab Plant. Mol. Physiol. Tokyo Univ. Fac. Pharmaceutical. Science. Nagoya City Univ.
-
Kim S
Univ. Tokyo Tokyo
-
Higuchi K
Laboratory Of Plant Production Chemistry Department Of Applied Biology And Chemistry Tokyo Universit
-
Nishizawa Naoko
Environmental Science Tohoku University
-
Kawasaki Shinji
National Inst. Of Agrobiological Sciences
-
Higuchi Kyoko
Tokyo Univ. Agriculture Tokyo Jpn
-
Mori S
Meijo Univ. Aichi Jpn
-
Nakanishi Hiromi
Laboratory Of Plant Molecular Physiology The University Of Tokyo
-
Takahashi Michiko
Laboratory Of Piant Molecular Physiology Department Of Applied Biological Chemistry The University O
-
Takahashi Michiko
Lab Plant. Mol. Physiol. Tokyo Univ. Fac. Pharmaceutical. Science. Nagoya City Univ.
-
Higuchi Kyoko
Laboratory Of Plant Production Chemistry Department Of Applied Biology And Chemistry Faculty Of Appl
-
Higuchi Kyoko
Laboratory Of Plant Production Chemistry Department Of Applied Biology And Chemistry Faculty Of Applied Bio-science Tokyo University Of Agriculture
-
Nishizawa Naoko-kishi
Laboratory Of Plant Molecular Physiology The University Of Tokyo
関連論文
- Pressure-Induced Superconductivity in Antiferromagnet CePd_5Al_2(Condensed matter: electronic structure and electrical, magnetic, and optical properties)
- Comparison of the functions of the barley nicotianamine synthase gene HvNAS1 and rice nicotianamine synthase gene OsNAS1 promoters in response to iron deficiency in transgenic tobacco(Plant Nutrition)
- Transgenic rice lines that include barley genes have increased tolerance to low iron availability in a calcareous paddy soil(Plant Nutrition)
- Avidin Expressed in Transgenic Rice Confers Resistance to the Stored-Product Insect Pests Tribolium confusum and Sitotroga cerealella
- Surperconductivity of CeRhIn_5 under High Pressure
- 10-1 GUS-staining analysis of the rice iron-deficiency-responsive element binding factors IDEF1 and IDEF2
- Synthesis of nicotianamine and deoxymugineic acid is regulated by OsIRO2 in Zn excess rice plants(Plant Nutrition)
- How are metal nutrients transported to the embryo in rice plant?
- Overcoming Fe deficiency in guava (Psidium guajava L.) by co-situs application of controlled release fertilizers(Fertilizer and Soil Amendments)
- ^Mn translocation in barley monitored using a positron-emitting tracer imaging system(Plant Nutrition)
- 9-22 Characterization of Glutathione transporter in rice
- Iron deficiency enhances cadmium uptake and translocation mediated by the Fe^ transporters OsIRT1 and OsIRT2 in rice(Plant Nutrition)
- Metabolic Engineering of Saccharomyces cerevisiae Producing Nicotianamine : Potential for Industrial Biosynthesis of a Novel Antihypertensive Substrate
- Construction of Artificial Promoters Highly Responsive to Iron Deficiency
- Directed Evolution of Yeast Ferric Reductase to Produce Plants with Tolerance to Iron Deficiency in Alkaline Soils
- A Rice FRD3-Like (OsFRDL1) Gene Is Expressed in the Cells Involved in Long-Distance Transport
- Diurnal Changes in the Expression of Genes That Participate in Phytosiderophore Synthesis in Rice
- H_2^O Translocation in Rice Was Enhanced by 10 μM 5-Aminolevulinic Acid as Monitored by Positron Emitting Tracer Imaging System (PETIS)
- Analysis of Upstream Region of Nicotianamine Synthase Gene from Arabidopsis thaliana : Presence of Putative ERE-like Sequence(Biochemistry & Molecular Biology)
- The Expression of a Barley HvNAS1 Nicotianamine Synthase Gene Promoter-gus Fusion Gene in Transgenic Tobacco is Induced by Fe-deficiency in Roots(Biochemistry & Molecular Biology)
- Analysis of Transgenic Rice Containing Barley Nicotianamine Synthase Gene
- ID17,A NEW IRON-REGULATED ABC TRANSPORTER FROM BARLEY ROOTS, LOCALIZED TO THE TONOPLAST
- ANALYSIS OF UPSTREAM REGION OF NICOTIANAMINE SYNTHASE GENES FROM Arabidopsis thaliana (II)
- Enhancement of nitrate reduction by chlorine application in Suaeda salsa (L.) Pall(Plant Nutrition)
- Effects of Anion Channel Blockers on Xylem Nitrate Transport in Barley Seedlings
- Absorption and Translocation of ^Fe, ^Mn and ^Zn Visualized by PETIS in Barley and Rice
- Visualization of ^O-Water Flow in Tomato and Rice in the Light and Dark Using a Positron-Emitting Tracer Imaging System (PETIS)
- Analysis of ^NH_4^+ uptake, translocation and retranslocation in rice by PETIS (Positron Emitting Tracer Imaging System)
- Absorption and Translocation of ^Mn Visualized by PETIS in Barley and Tomato
- P11-12 Low cadmium (LCD), a novel gene related to cadmium tolerance and accumulation in rice seeds
- Technical Advance: An Automated Device for Cryofixation of Specimens of Electron Microscopy using Liquid Helium
- AUTOMATED DEVICE FOR CRYOFIXATION OF ELECTRON MICROSCOPIC SPECIMEN AT LIQUID HELIUM TEMPERATURE
- Importance of contact between rice roots and co-situs applied fertilizer granules on iron absorption by paddy rice in a calcareous paddy soil(Fertilizers and Soil Amendments)
- A Proteinase-Storing Body that Prepares for Cell Death or Stresses in the Epidermal Cells of Arabidopsis
- A NOVEL LARGE BODY THAT IS DERIVED FROM ENDOPLASMIC RETICULUM
- Establishment of a Cell-Free System for the Biosynthesis of Nicotianamine
- Expression of a Gene Specific for Iron Deficiency (Ids3) in the Roots of Hordeum vulgare
- Construction of a BAC Library of the Rice Blast Fungus Magnaporthe grisea and Finding Specific Genome Regions in which Its Transposons Tend to Cluster
- CHARACTERIZATION OF TWO PROTEINS INDUCED BY IRON DEFICIENCY IN BARLEY ROOTS
- STUDY ON THE BIOSYNTHETIC PATHWAY OF MUGINEIC ACID IN Fe-DEFICIENT BARLEY ROOTS V : PURIFICATION OF NICOTIANAMINE AMINOTRANSFERASE
- Construction of a saturated genome map model legume Lotus japonicus using HEGS (high efficiency genome scanning) in half a year
- Seismological and geological characterization of the crust in the southern part of northern Fossa Magna, central Japan
- Induction of Adenine Phosphoribosyltransferase (APRT) in Iron-deficient Barley Roots
- Biosynthetic Pathway of Phytosiderophores in Iron-Deficient Graminaceous Plants : Development of an Assay System for the Detection of Nicotianamine Aminotransferase Activity
- Cross-Reactivities of Anti-Nicotianamine Antibody with Mugineic Acid and Related Compounds
- Enhancement of Superconducting Transition Temperature in CeCu_2Ge_2 under High Pressures
- Exotic Superconductivity in the Coexistent Phase of Antiferromagnetism and Superconductivity in CeCu_2(Si_Ge_)_2 : A Cu-NQR Study under Hydrostatic Pressure(Condensed Matter : Electronic Structure, Electrical, Magnetic and Optical Properties)
- Exotic Superconductivity in the Coexistent Phase of Antiferromagnetism and Superconductivity in CeCu_2(Si_Ge_)_2 : A Cu-NQR Study under Hydrostatic Pressure
- Water (H_2 ^15O) Flow in Rice is Regulated by the Concentration of Nutrients as Monitored by Positron Multi-Probe System (PMPS)
- Anisotropic Spin Fluctuations in Heavy-Fermion Superconductor CeCoIn_5 : In-NQR and Co-NMR Studies
- Anisotropic Spin Fluctuations in Heavy-Fermion Superconductor CeCoIn_5 : In-NQR and Co-NMR Studies
- Evolution of the Magnetism and Superconductivity in CeRhIn5 under Pressure: 115In-NQR Study (Proceedings of the International Conference on Strongly Correlated Electrons with Orbital Degrees of Freedom(ORBITAL2001))
- Genomic Organization of the S-Locus Region of Brassica(Biochemistry & Molecular Biology)
- IN-VIVO IDENTIFICATION OF MUGINEIC ACID SYNTHASE GENE USING TRANSGENIC RICE
- Induction of the IDI1 Gene in Fe-Deficient Barley Roots: A Gene Encoding a Putative Enzyme That Catalyses the Methionine Salvage Pathway for Phytosiderophore Production
- ISOLATION OF A cDNA FOR AN FE-DEFICIENCY INDUCIBLE LIPOXYGENASE ISOFORM FROM BARLEY
- ANALYSIS OF UPSTREAM REGION OF NICOTIANAMINE SYNTHASE GENES FROM Arabidopsis thaliana
- TRANSGENIC RICE AND TOBACCO CONTAINING CaMV35S-NICOTIANAMINE SYNTHASE GENE (HVNAS1)
- THE TOBACCO OVEREXPRESSED WITH NAAT-A GENE SHOWED INTERCOSTAL CHLOROSIS
- Cloning of Nicotianamine Synthase Genes from Arabidopsis thaliana
- Time Course Study of Aluminum-Induced Callose Formation in Barley Roots as observed by Digital Microscopy and Low-Vacuum Scanning Electron Microscopy
- Presence of Nicotianamine Synthase Isozymes and Their Homologues in the Root of Graminaceous Plants
- Conforcal Images of Adenine Phosphoribosyltransferase (APRT) Fused to sGFP in Plant Cells
- THE FUNCTIONAL ANALYSIS OF BARLEY SFD1 GENE, A SUPPRESSOR OF YEAST COPPER UPTAKE MUTANT ctr 1
- CLONING AND CHARACTERIZATION OF NICOTIANAMINE SNTHASE GENES FROM Arabidopsis thaliana
- CLONING OF NICOTIANAMINE SYNTHASE GENES (nas) FROM GRAMINACEOUS PLANTS
- DEVELOPMENT OF THE TRANSGENIC RICE OVEREXPRESSING NICOTIANAMINE AMINOTRANSFERASE
- Characterizing Nicotianamine Aminotransferase : Improving Its Assay System and Details of the Regulation of Its Activity by Fe Nutrition Status
- THE FUNCTIONAL ANALYSIS OF SFD1 GENE, WHICH RESTORES THE MUTATION OF YEAST COPPER UPTAKE MUTANT ctr1
- STUDY ON THE BIOSYNTHETIC PATHWAY OF MUGINEIC ACID IN Fe-DEFICIENT BARLEY ROOTS VI : SEQUENCE AND EXPRESSION OF NICOTIANAMINE AMINOTRANSFERASE GENE
- Co-situs Application of Controlled-Release Fertilizers to Alleviate Iron Chlorosis of Paddy Rice Grown in Calcareous Soil
- Invagination of plasmalemma : Its role in the absorption of macromolecules in rice roots
- A CELL PLATE-LOCALIZED ARABIDOPSIS ENDO-1,4-β-GLUCANASE IS ESSENTIAL FOR CYTOKINESIS
- VACUOLE FORMATION AS A RESULT OF INTRACELLULAR DIGESTION : ACID PHOSPHATASE LOCALIZATION AS ASSOCIATED WITH PLASMALEMMA-INVAGINATION AND VACUOLE FORMATION
- Endocytosis (heterophagy) in plant cells : Involvement of ER and ER-derived vesicles
- Electronmicroscope-autoradiographical evidence for the incorporation of exogenous protein into rice root cells
- Incidence of thiophanate-methyl resistance in Cercospora kikuchii within a single lineage based on amplified fragment length polymorphisms in Japan
- ALLEVIATION EFFECT OF ARGININE ON ARTIFICIALLY REDUCED GRAIN YIELD OF NH_4^+-OR NO_3^--FED RICE
- Synthesis of Arabinose-Containing Cell Wall Precursors in Suspension-Cultured Tobacco Cells III. Purification and Some Properties of the Major Component
- A Vesicle Containing Fibrous Materials Appearing in Water-Stressed Corn Root Cells
- QTL Analysis for Resistance to Phytophthora Blight (Phytophthora capsici Leon.) Using an Intraspecific Doubled-Haploid Population of Capsicum annuum
- Rapid Construction of a Linkage Map using High-efficiency Genome Scanning/AFLP and RAPD, Based on an Intraspecific, Doubled-haploid Population of Capsicum annuum
- Changes in levels of Proteases and Their Inhibitors during Development of the Seeds of Job's Tears (Coix lacryma-jobi L. var. Ma-yuen Stapf) and Their Localization and Interactions
- ^Fe Translocation in Barley as Monitored by a Positron-Emitting Tracer Imaging System (PETIS) : Evidence for the Direct Translocation of Fe from Roots to Young Leaves via Phloem
- A Highly Sensitive, Quick and Simple Quantification Method for Nicotianamine and 2'-Deoxymugineic Acid from Minimum Samples Using LC/ESI-TOF-MS Achieves Functional Analysis of These Components in Plants
- Deep seismic reflection profiling across the Northern Fossa Magna : The ERI 1997 and the JNOC 1996 seismic lines, active faults and geological structures
- O-152 Deep Seismic Reflection Profiling across the Northern Fossa Magna : the ERI 1997 and the JNOC 1996 Seismic Lines, Active Faults and Geological Structure model
- NICOTIANAMINE-IRON (NA-FE) IS REQUIRED FOR THE FORMATION OF NORMAL INFLORESCENCE AND SEEDS' MATURATION
- 10-13 ミャンマーのイネ品種の種子における微量栄養素の解析(10.植物の微量栄養素,2010年度北海道大会)
- PRIMARY ASSIMILATION PROCESS OF TRIPLY (^N, ^C and ^3H) LABELED ARGININE IN THE ROOTS OF ARGININE-FED BARLEY
- Comparison of Iron Availability in Leaves of Barley and Rice(Plant Nutrition)
- Construction and Characterization of a Bacterial Artificial Chromosome (BAC) Library from the Japanese Malting Barley variety 'Haruna Nijo'
- OCCURRENCE OF STRESS GRAINS IN THE ROOT CELLS OF BROAD BEAN AND BARLEY PLANTS UNDER DESICCATION CONDITIONS
- Highly Sensitive Quantitative Analysis of Nicotianamine Using LC/ESI-TOF-MS with an Internal Standard
- Light-Dependent Iron Transport into Isolated Barley Chloroplasts
- Characteristics of Na[+] and K[+] absorption in Suaeda salsa (L.) Pall
- CLONING OF SAM SYNTHETASE GENES AND THEIR EXPRESSION IN BARLEY ROOTS UNDER VARIOUS STRESS CONDITIONS.
- Reevaluation of the genes induced by iron deficiency in barley roots
- Rice phenolics efflux transporter 2 (PEZ2) plays an important role in solubilizing apoplasmic iron(Plant nutrition)