Changes in levels of Proteases and Their Inhibitors during Development of the Seeds of Job's Tears (Coix lacryma-jobi L. var. Ma-yuen Stapf) and Their Localization and Interactions

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Changes in levels and localization of proteases and a trypsin inhibitor (JBTI) in the developing seeds of Job's tears (Coix lacryma-jobi L. var. Ma-yuen Stapf) were followed, and their interactions were examined. The JBTI was induced from the middle stage of development (12 DAF, i. e, 12 days after flowering) and increased until the late stage of development (24 DAF), and was localized in the germ. Two groups of proteases (A, those with molecular weights of 55-70 kDa; and B, those with molecular weights greater than 94 kDa) were detected by active staining of substrate-containing SDS-polyacrylamide gels. The group of larger proteases seems to consist of three members (B_1, B_2, B_3, with increasing molecular weights). Band A was observed only in the early stage of development (until DAF 9), band B_2 persisted during all stages, while bands B_1 and B_3 were present only during early and late stages, respectively. The group A proteases and one of the group B proteases (probably B_1) were inhibited by diisopropyl-fluorophosphate (DFP), by trypsin inhibitors from soybean and rice, and by JBTI. The proteases that were present in the seeds of Job's tears at a late stage seemed to be localized in the germ.
日本植物生理学会の論文