QTL Analysis for Resistance to Phytophthora Blight (Phytophthora capsici Leon.) Using an Intraspecific Doubled-Haploid Population of Capsicum annuum
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概要
- 論文の詳細を見る
A doubled-haploid (DH) population (n=176) obtained by anther culture of an F_1 hybrid between a line susceptible to Phytophthora capsici 'K9-11' (Capsicum annuum L.) and a line resistant to P. capsici 'AC2258' (C. annuum L.) was inoculated with P. capsici. QTL analysis of the resistance was performed using a linkage map consisting of 16 linkage groups (LGs), covering a total distance of 1100.5cM. Three QTLs were detected on LG1, LG6 and LG7. The QTL with the highest LOD score, detected on LG7, explained 82.7% of the phenotypic variance with a LOD score of 67.02. This QTL was designated as Phyt-1. The nearest marker was an AFLP marker, M10E3-6. The second QTL, designated as Phyt-2, was found on LG1. It explained 6.4% of the phenotypic variance with a LOD score of 2.54. The nearest RAPD marker was RP13-1. The other QTL, designated as Phyt-3, which was found on LG6, explained 5.6% of the phenotypic variance with a LOD score of 2.20. The nearest AFLP marker was M9E3-11. It was confirmed that the lines with a high resistance could be efficiently selected by using two markers, M10E3-6 and RP13-1, simultaneously. The presence of both Phyt-1 and Phyt-2 under homozygous conditions may enable to breed resistant cultivars of sweet pepper. The molecular markers identified in the present study could be useful for marker-assisted selection (MAS) in order to breed sweet pepper cultivars with a high resistance to P. capsici using 'AC2258' as a source of resistance genes.
- 日本育種学会の論文
著者
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KAWASAKI Shinji
National Institute of Agrobiological Sciences
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NAGATA Ryutaro
Miyazaki Prefectural Agricultural Experiment Station
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Kinoshita Takehito
Laboratory Of Plant Genetics And Breeding Faculty Of Agriculture Saga University:(present Office)sag
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Yamaguchi Kazunori
Miyazaki Prefectural Agricultural Experiment Station:(present Address)miyazaki Prefectural Chubu Agr
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Nagata R
Miyazaki Prefectural Agricultural Experiment Station:(present Address)miyazaki Prefectural Agricultu
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Kinoshita T
Laboratory Of Plant Genetics And Breeding Faculty Of Agriculture Saga University:(present Office)sag
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Sugita Toru
Miyazaki Prefectural Agricultural Experiment Station
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Kinoshita Tetsuji
Miyazaki Prefectural Agricultural Experiment Station
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Yuji Kenichi
Miyazaki Prefectural Agricultural Experiment Station
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Sugimura Yukiyo
Miyazaki Prefectural Agricultural Experiment Station
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Todoroki Atsushi
Miyazaki Prefectural Agricultural Experiment Station
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Kawasaki Shinji
National Inst. Of Agrobiological Sciences
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Sugimura Yukiyo
Miyazaki Prefectural Agricultural Experiment Station:(present Address)miyazaki Prefectural Governmen
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Yuji Kenichi
Miyazaki Prefectural Agricultural Experiment Station:(present Address)miyazaki Prefectural Koyu Agri
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