Development of SCAR Markers Linked to L^3 gene in Capsicum
スポンサーリンク
概要
- 論文の詳細を見る
Random amplified polymorphic DNA (RAPD) markers linked to the L^3 locus were developed by applying the bulked segregant analysis method to two doubled haploid (DH) populations in Capsicum. The codominant RAPD markers, E18_<272> and E18_<286>, were converted into sequence-characterized amplified region (SCAR) markers by molecular cloning and nucleotide sequencing. A PCR analysis using DH (n = 176) and backcross (n = 190) populations revealed that all the SCAR markers, PMFR11_<269>, PMFR11_<283> and PMFR21_<200>, co-segregated with the original RAPD markers, and were mapped at a distance of 4.0 cM from the L^3 locus. Furthermore, after confirmation of their validity in 18 accessions of Capsicum spp. using the codominant SCAR marker pair, PMFR11_<269> and PMFR11_<283>, it was suggested that the SCAR markers developed here could become effective in marker-assisted selection programs for the introduction of the L^3 gene derived from PI 159236 (C. chinense) into sweet pepper for breeding purposes.
- 日本育種学会の論文
著者
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NAGATA Ryutaro
Miyazaki Prefectural Agricultural Experiment Station
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Kinoshita Takehito
Laboratory Of Plant Genetics And Breeding Faculty Of Agriculture Saga University:(present Office)sag
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Yamaguchi Kazunori
Miyazaki Prefectural Agricultural Experiment Station:(present Address)miyazaki Prefectural Chubu Agr
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Nagata R
Miyazaki Prefectural Agricultural Experiment Station:(present Address)miyazaki Prefectural Agricultu
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Kinoshita T
Laboratory Of Plant Genetics And Breeding Faculty Of Agriculture Saga University:(present Office)sag
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Sugita Toru
Miyazaki Prefectural Agricultural Experiment Station
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Kinoshita Tetsuji
Miyazaki Prefectural Agricultural Experiment Station
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Yuji Kenichi
Miyazaki Prefectural Agricultural Experiment Station
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Sugimura Yukiyo
Miyazaki Prefectural Agricultural Experiment Station
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Todoroki Atsushi
Miyazaki Prefectural Agricultural Experiment Station
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Sugimura Yukiyo
Miyazaki Prefectural Agricultural Experiment Station:(present Address)miyazaki Prefectural Governmen
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Yuji Kenichi
Miyazaki Prefectural Agricultural Experiment Station:(present Address)miyazaki Prefectural Koyu Agri
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