Comparison of the functions of the barley nicotianamine synthase gene HvNAS1 and rice nicotianamine synthase gene OsNAS1 promoters in response to iron deficiency in transgenic tobacco(Plant Nutrition)

スポンサーリンク

概要

• 論文の詳細を見る

• Barley (Hordeum vulgare L.) nicotianamine synthase gene (HvNAS1) expression in barley is strongly induced by Fe deficiency in the roots and rice (Oryza sativa L.) nicotianamine synthase gene (OsNAS1) expression in rice is induced by Fe deficiency both in the roots and in the shoots. In dicots, NAS genes are not strongly induced by Fe deficiency, and they function to maintain Fe homeostasis. Rice OsNAS1promoter::GUS or barley HvNAS1promoter::GUS was introduced into tobacco (Nicotiana tabacum L.) and tissue specificities and systemic regulation of their expression were compared. A split-root experiment revealed that the HvNAS1 promoter exhibited functions similar to those of Fe-acquisition-related genes in tobacco roots, suggesting that this promoter responded to certain Fe-deficiency systemic signals and to the Fe concentration in the rhizosphere. The HvNAS1 promoter might harbor a type of universal system of gene expression for Fe acquisition. However, the OsNAS1 promoter did not respond to local application of Fe to the roots and induced GUS activities in mature leaves in response to Fe deficiency. This promoter might possess numerous types of cis-acting sequences that are involved in Fe metabolism.

著者

• Nishizawa Naoko

  Laboratory Of Plant Biotechnology Graduate School Of Agricultural And Life Sciences The University O

• Mori S

  Laboratory Of Plant Biotechnology Graduate School Of Agricultural And Life Sciences The University O

• ITO Satoshi

  Laboratory of Plant Production Chemistry, Department of Applied Biology and Chemistry, Tokyo Univers

• INOUE Haruhiko

  Laboratory of Plant Biotechnology, Graduate School of Agricultural and Life Sciences, The University

• KOBAYASHI Takanori

  Laboratory of Plant Biotechnology, Graduate School of Agricultural and Life Sciences, The University

• YOSHIBA Masaaki

  Laboratory of Plant Production Chemistry, Department of Applied Biology and Chemistry, Tokyo Univers

• MORI Satoshi

  Laboratory of Plant Biotechnology, Graduate School of Agricultural and Life Sciences, The University

• HIGUCHI Kyoko

  Laboratory of Plant Production Chemistry, Department of Applied Biology and Chemistry, Tokyo Univers

• Matsuhashi S

  Takasaki Advanced Radiation Research Institute Japan Atomic Energy Agency

• Nishizawa Naoko

  The University Of Tokyo

• Nishizawa Naoko-kishi

  Lab Plant. Mol. Physiol. Tokyo Univ. Fac. Pharmaceutical. Science. Nagoya City Univ.

• Mori S

  Dept. Of Appl. Biol. Chem. Univ. Tokyo

• Mori Satoshi

  Laboratory Of Piant Molecular Physiology Department Of Applied Biological Chemistry The University O

• Kobayashi Takanori

  Univ. Tokyo Tokyo

• Mori Satoshi

  Lab Plant. Mol. Physiol. Tokyo Univ. Fac. Pharmaceutical. Science. Nagoya City Univ.

• Nishizawa N‐k

  Univ. Tokyo Tokyo Jpn

• Mori S

  Department Of Applied Biological Chemistry The University Of Tokyo

• Higuchi K

  Laboratory Of Plant Production Chemistry Department Of Applied Biology And Chemistry Tokyo Universit

• Inoue Haruhiko

  Laboratory Of Plant Biotechnology Graduate School Of Agricultural And Life Sciences The University O

• Nishizawa Naoko

  Environmental Science Tohoku University

• Higuchi Kyoko

  Tokyo Univ. Agriculture Tokyo Jpn

• Mori S

  Meijo Univ. Aichi Jpn

• Kobayashi Takanori

  東大農

• Ito Satoshi

  Laboratory Of Plant Production Chemistry Department Of Applied Biology And Chemistry Tokyo Universit

• Ito Satoshi

  Laboratoly Of Nutritional Chemistry Faculty Of Nutrition And High Technology Research Center Kobe-ga

• Nishizawa Naoko-kishi

  Laboratory Of Plant Nutrition And Fertilizers Department Of Agricultural Chemistry The University Of

• Yoshiba Masaaki

  Laboratory Of Plant Production Chemistry Department Of Applied Biology And Chemistry Tokyo Universit

• Yoshiba Masaaki

  Laboratory Of Plant Production Chemistry Department Of Applied Biology And Chemistry Faculty Of Appl

• Higuchi Kyoko

  Laboratory Of Plant Production Chemistry Department Of Applied Biology And Chemistry Faculty Of Appl

• Higuchi Kyoko

  Laboratory Of Plant Production Chemistry Department Of Applied Biology And Chemistry Faculty Of Applied Bio-science Tokyo University Of Agriculture

• Nishizawa Naoko-kishi

  Laboratory Of Plant Molecular Physiology The University Of Tokyo

関連論文

• Comparison of the functions of the barley nicotianamine synthase gene HvNAS1 and rice nicotianamine synthase gene OsNAS1 promoters in response to iron deficiency in transgenic tobacco(Plant Nutrition)
• Transgenic rice lines that include barley genes have increased tolerance to low iron availability in a calcareous paddy soil(Plant Nutrition)
• Detection and Characterization of Protein Kinases in Rice Phloem Sap : ENVIRONMENTAL AND STRESS RESPONSES : PROTEINS, ENZYMES AND METABOLISM
• 10-1 GUS-staining analysis of the rice iron-deficiency-responsive element binding factors IDEF1 and IDEF2
• Synthesis of nicotianamine and deoxymugineic acid is regulated by OsIRO2 in Zn excess rice plants(Plant Nutrition)
• How are metal nutrients transported to the embryo in rice plant?
• Overcoming Fe deficiency in guava (Psidium guajava L.) by co-situs application of controlled release fertilizers(Fertilizer and Soil Amendments)
• ^Mn translocation in barley monitored using a positron-emitting tracer imaging system(Plant Nutrition)
• 9-22 Characterization of Glutathione transporter in rice
• Iron deficiency enhances cadmium uptake and translocation mediated by the Fe^ transporters OsIRT1 and OsIRT2 in rice(Plant Nutrition)
• Metabolic Engineering of Saccharomyces cerevisiae Producing Nicotianamine : Potential for Industrial Biosynthesis of a Novel Antihypertensive Substrate
• Construction of Artificial Promoters Highly Responsive to Iron Deficiency
• Directed Evolution of Yeast Ferric Reductase to Produce Plants with Tolerance to Iron Deficiency in Alkaline Soils
• A Rice FRD3-Like (OsFRDL1) Gene Is Expressed in the Cells Involved in Long-Distance Transport
• Diurnal Changes in the Expression of Genes That Participate in Phytosiderophore Synthesis in Rice
• H_2^O Translocation in Rice Was Enhanced by 10 μM 5-Aminolevulinic Acid as Monitored by Positron Emitting Tracer Imaging System (PETIS)
• Analysis of Upstream Region of Nicotianamine Synthase Gene from Arabidopsis thaliana : Presence of Putative ERE-like Sequence(Biochemistry & Molecular Biology)
• The Expression of a Barley HvNAS1 Nicotianamine Synthase Gene Promoter-gus Fusion Gene in Transgenic Tobacco is Induced by Fe-deficiency in Roots(Biochemistry & Molecular Biology)
• Analysis of Transgenic Rice Containing Barley Nicotianamine Synthase Gene
• ID17,A NEW IRON-REGULATED ABC TRANSPORTER FROM BARLEY ROOTS, LOCALIZED TO THE TONOPLAST
• ANALYSIS OF UPSTREAM REGION OF NICOTIANAMINE SYNTHASE GENES FROM Arabidopsis thaliana (II)
• Protein Phosphorylation in the Sieve Tubes of Rice Plants
• Enhancement of nitrate reduction by chlorine application in Suaeda salsa (L.) Pall(Plant Nutrition)
• Growth response of Suaeda salsa (L.) Pall to graded NaCl concentrations and the role of chlorine in growth stimulation(Plant Nutrition)
• Responses of Ganoderma lucidum to heavy metals
• Growth and fruitbody formation of Ganoderma Iucidum on media supplemented with vanadium, selenium and germanium
• Effects of Anion Channel Blockers on Xylem Nitrate Transport in Barley Seedlings
• Absorption and Translocation of ^Fe, ^Mn and ^Zn Visualized by PETIS in Barley and Rice
• Visualization of ^O-Water Flow in Tomato and Rice in the Light and Dark Using a Positron-Emitting Tracer Imaging System (PETIS)
• Analysis of ^NH_4^+ uptake, translocation and retranslocation in rice by PETIS (Positron Emitting Tracer Imaging System)
• Absorption and Translocation of ^Mn Visualized by PETIS in Barley and Tomato
• Translocation of ^C-Methionine Visualized by Positron Emitting Tracer Imaging System (PETIS) in Barley
• P11-12 Low cadmium (LCD), a novel gene related to cadmium tolerance and accumulation in rice seeds
• Technical Advance: An Automated Device for Cryofixation of Specimens of Electron Microscopy using Liquid Helium
• AUTOMATED DEVICE FOR CRYOFIXATION OF ELECTRON MICROSCOPIC SPECIMEN AT LIQUID HELIUM TEMPERATURE
• Importance of contact between rice roots and co-situs applied fertilizer granules on iron absorption by paddy rice in a calcareous paddy soil(Fertilizers and Soil Amendments)
• A Proteinase-Storing Body that Prepares for Cell Death or Stresses in the Epidermal Cells of Arabidopsis
• A NOVEL LARGE BODY THAT IS DERIVED FROM ENDOPLASMIC RETICULUM
• Establishment of a Cell-Free System for the Biosynthesis of Nicotianamine
• Expression of a Gene Specific for Iron Deficiency (Ids3) in the Roots of Hordeum vulgare
• CHARACTERIZATION OF TWO PROTEINS INDUCED BY IRON DEFICIENCY IN BARLEY ROOTS
• STUDY ON THE BIOSYNTHETIC PATHWAY OF MUGINEIC ACID IN Fe-DEFICIENT BARLEY ROOTS V : PURIFICATION OF NICOTIANAMINE AMINOTRANSFERASE
• Induction of Adenine Phosphoribosyltransferase (APRT) in Iron-deficient Barley Roots
• Biosynthetic Pathway of Phytosiderophores in Iron-Deficient Graminaceous Plants : Development of an Assay System for the Detection of Nicotianamine Aminotransferase Activity
• Development and Characterization of a Monoclonal Antibody to Phytosiderophores
• Cross-Reactivities of Anti-Nicotianamine Antibody with Mugineic Acid and Related Compounds
• Influence of Dietary Protein Levels on β-Alanine Aminotransferase Expression and Activity in Rats
• Water (H_2 ^15O) Flow in Rice is Regulated by the Concentration of Nutrients as Monitored by Positron Multi-Probe System (PMPS)
• Recent insights into iron homeostasis and their application in graminaceous crops
• IN-VIVO IDENTIFICATION OF MUGINEIC ACID SYNTHASE GENE USING TRANSGENIC RICE
• Induction of the IDI1 Gene in Fe-Deficient Barley Roots: A Gene Encoding a Putative Enzyme That Catalyses the Methionine Salvage Pathway for Phytosiderophore Production
• ISOLATION OF A cDNA FOR AN FE-DEFICIENCY INDUCIBLE LIPOXYGENASE ISOFORM FROM BARLEY
• ANALYSIS OF UPSTREAM REGION OF NICOTIANAMINE SYNTHASE GENES FROM Arabidopsis thaliana
• TRANSGENIC RICE AND TOBACCO CONTAINING CaMV35S-NICOTIANAMINE SYNTHASE GENE (HVNAS1)
• THE TOBACCO OVEREXPRESSED WITH NAAT-A GENE SHOWED INTERCOSTAL CHLOROSIS
• Cloning of Nicotianamine Synthase Genes from Arabidopsis thaliana
• Time Course Study of Aluminum-Induced Callose Formation in Barley Roots as observed by Digital Microscopy and Low-Vacuum Scanning Electron Microscopy
• Presence of Nicotianamine Synthase Isozymes and Their Homologues in the Root of Graminaceous Plants
• Conforcal Images of Adenine Phosphoribosyltransferase (APRT) Fused to sGFP in Plant Cells
• THE FUNCTIONAL ANALYSIS OF BARLEY SFD1 GENE, A SUPPRESSOR OF YEAST COPPER UPTAKE MUTANT ctr 1
• CLONING AND CHARACTERIZATION OF NICOTIANAMINE SNTHASE GENES FROM Arabidopsis thaliana
• CLONING OF NICOTIANAMINE SYNTHASE GENES (nas) FROM GRAMINACEOUS PLANTS
• DEVELOPMENT OF THE TRANSGENIC RICE OVEREXPRESSING NICOTIANAMINE AMINOTRANSFERASE
• Characterizing Nicotianamine Aminotransferase : Improving Its Assay System and Details of the Regulation of Its Activity by Fe Nutrition Status
• THE FUNCTIONAL ANALYSIS OF SFD1 GENE, WHICH RESTORES THE MUTATION OF YEAST COPPER UPTAKE MUTANT ctr1
• STUDY ON THE BIOSYNTHETIC PATHWAY OF MUGINEIC ACID IN Fe-DEFICIENT BARLEY ROOTS VI : SEQUENCE AND EXPRESSION OF NICOTIANAMINE AMINOTRANSFERASE GENE
• Difference in Al Sensitivity between Seminal and Crown Roots of Rice Nursery Seedlings (Plant Nutrition)
• Extraordinary High Aluminium Tolerance of the Acidophilic Thermophilic Alga, Cyanidium caldarium
• Co-situs Application of Controlled-Release Fertilizers to Alleviate Iron Chlorosis of Paddy Rice Grown in Calcareous Soil
• Invagination of plasmalemma : Its role in the absorption of macromolecules in rice roots
• A CELL PLATE-LOCALIZED ARABIDOPSIS ENDO-1,4-β-GLUCANASE IS ESSENTIAL FOR CYTOKINESIS
• VACUOLE FORMATION AS A RESULT OF INTRACELLULAR DIGESTION : ACID PHOSPHATASE LOCALIZATION AS ASSOCIATED WITH PLASMALEMMA-INVAGINATION AND VACUOLE FORMATION
• Endocytosis (heterophagy) in plant cells : Involvement of ER and ER-derived vesicles
• Electronmicroscope-autoradiographical evidence for the incorporation of exogenous protein into rice root cells
• Identification of Barley Chromosome No.4, Possible Encoder of Genes of Mugineic acid Synthesis from 2'-Deoxymugineic acid Using Wheat-Barley Addition Lines
• ALLEVIATION EFFECT OF ARGININE ON ARTIFICIALLY REDUCED GRAIN YIELD OF NH_4^+-OR NO_3^--FED RICE
• A Vesicle Containing Fibrous Materials Appearing in Water-Stressed Corn Root Cells
• ^Fe Translocation in Barley as Monitored by a Positron-Emitting Tracer Imaging System (PETIS) : Evidence for the Direct Translocation of Fe from Roots to Young Leaves via Phloem
• A Highly Sensitive, Quick and Simple Quantification Method for Nicotianamine and 2'-Deoxymugineic Acid from Minimum Samples Using LC/ESI-TOF-MS Achieves Functional Analysis of These Components in Plants
• Glucagon-like peptide-1 inhibits LPS-induced IL-1β production in cultured rat astrocytes
• NICOTIANAMINE-IRON (NA-FE) IS REQUIRED FOR THE FORMATION OF NORMAL INFLORESCENCE AND SEEDS' MATURATION
• 10-13 ミャンマーのイネ品種の種子における微量栄養素の解析(10.植物の微量栄養素,2010年度北海道大会)
• PRIMARY ASSIMILATION PROCESS OF TRIPLY (^N, ^C and ^3H) LABELED ARGININE IN THE ROOTS OF ARGININE-FED BARLEY
• Comparison of Iron Availability in Leaves of Barley and Rice(Plant Nutrition)
• OCCURRENCE OF STRESS GRAINS IN THE ROOT CELLS OF BROAD BEAN AND BARLEY PLANTS UNDER DESICCATION CONDITIONS
• Highly Sensitive Quantitative Analysis of Nicotianamine Using LC/ESI-TOF-MS with an Internal Standard
• Iron deficiency causes zinc excess in Zea mays(Plant Nutrition)
• Contribution of iron associated with high-molecular-weight substances to the maintenance of the SPAD value of young leaves of barley under iron-deficient conditions(Plant Nutrition)
• Remodeling of the Major Light-Harvesting Antenna Protein of PSII Protects the Young Leaves of Barley (Hordeum vulgare L.) from Photoinhibition under Prolonged Iron Deficiency
• Regulation of paracellular barrier function in mouse submandibular gland acini
• CHARACTERIAZATION AND ANALYSIS OF ROMATE DEHYDROGENASE GENE : SITE SPECIFIC EXPRESSION IN IRON-DEFICIENT BARLEY ROOT
• Light-Dependent Iron Transport into Isolated Barley Chloroplasts
• Characteristics of Na[+] and K[+] absorption in Suaeda salsa (L.) Pall
• CLONING OF SAM SYNTHETASE GENES AND THEIR EXPRESSION IN BARLEY ROOTS UNDER VARIOUS STRESS CONDITIONS.
• Analysis of the iron deficiency-responsive expression of lds2; The 5'-deletion analysis of the promoter in Tobacco
• Modulation of macronutrient metabolism in barley leaves under iron-deficient condition(Plant Nutrition)
• Reevaluation of the genes induced by iron deficiency in barley roots
• Rice phenolics efflux transporter 2 (PEZ2) plays an important role in solubilizing apoplasmic iron(Plant nutrition)
• Characteristics of Na^+ and K^+ absorption in Suaeda salsa (L.) Pall.(Plant Nutrition)

もっと見る 閉じる

スポンサーリンク