Cloning of Nicotianamine Synthase Genes from Arabidopsis thaliana

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概要

• 論文の詳細を見る

• Nicotianamine synthase (NAS) catalyzes the trimerization of S-adenosylmethionine to form one molecule of nicotianamine (NA). NA is present in all the plants; it chelates metal cations, and is considered to play a role in metal homeostasis in plants. Moreover, in graminaceous monocotyledonous plants, NA is an essential intermediate in the biosynthesis of mugineic acid family phytosiderophores (MAs). In order to identify the gene encoding NAS in dicotyledonous plants, Arabidopsis thaliana databases were searched using the nucleotide sequence of the NAS gene from barley (HvNAS), which we have recently isolated. We found several ESTs and three genomic sequences highly homologous to HvNAS in the databases. Based on these nucleotide sequences and that of HvNAS, we designed 2 sets of primers to isolate the NAS orthologues in Arabidopsis and succeeded in obtaining three DNA clones encoding AtNAS (AtNAS1, 2, and 3). These clones were expressed in Escherichia coli and their protein products displayed the NAS activity. The expression of AtNAS1 was detected in both shoots and roots of A. thaliana by RT-PCR; AtNAS3 expression was only detected in the shoots. In contrast, AtNAS2 expression was not detected in any organs.

• 社団法人日本土壌肥料学会の論文

著者

• Nishizawa Naoko

  Laboratory Of Plant Biotechnology Graduate School Of Agricultural And Life Sciences The University O

• MORI Satoshi

  Laboratory of Plant Biotechnology, Graduate School of Agricultural and Life Sciences, The University

• NAKANISHI Hiromi

  Laboratory of Plant Molecular Physiology, Department of Applied Biological Chemistry, The University

• Suzuki Kiyonori

  Department Of Materials Science Kitami Institute Of Technology

• Nishizawa Naoko-kishi

  Lab Plant. Mol. Physiol. Tokyo Univ. Fac. Pharmaceutical. Science. Nagoya City Univ.

• Mori Satoshi

  Laboratory Of Piant Molecular Physiology Department Of Applied Biological Chemistry The University O

• Mori Satoshi

  Lab Plant. Mol. Physiol. Tokyo Univ. Fac. Pharmaceutical. Science. Nagoya City Univ.

• Suzuki Kazuya

  CREST, Japan Science and Technology Corporation (JST)

• Higuchi Kyoko

  CREST, Japan Science and Technology Corporation (JST)

• Higuchi K

  Laboratory Of Plant Production Chemistry Department Of Applied Biology And Chemistry Tokyo Universit

• Nishizawa Naoko

  Environmental Science Tohoku University

• Higuchi Kyoko

  Tokyo Univ. Agriculture Tokyo Jpn

• Mori S

  Meijo Univ. Aichi Jpn

• Nakanishi Hiromi

  Laboratory Of Plant Molecular Physiology The University Of Tokyo

• Higuchi Kyoko

  Laboratory Of Plant Production Chemistry Department Of Applied Biology And Chemistry Faculty Of Applied Bio-science Tokyo University Of Agriculture

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