Presence of Nicotianamine Synthase Isozymes and Their Homologues in the Root of Graminaceous Plants

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概要

• 論文の詳細を見る

• Nicotianamine synthase (NAS) catalyzes the synthesis of nicotianamine, which is an intermediate in the biosynthetic pathway of mugineie acid family phyto-siderophores (MAs). Using polyclonal anti-NAS antibodies and recombinant NAS proteins, we identified five NAS isozymes and one NAS homologue in Fe-deficient barley roots using two-dimensional electrophoresis followed by Western blot analysis. Other unidentified NAS homologues that were induced by Fe-deficiency were also detected in barley roots. Western analysis enabled to detect NAS homologues in wheat, oats, rice, maize, and sorghum roots. In graminaceous species, both the amount and number of NAS homologues were correlated with the total NAS activity and Fe-deficiency tolerance. The NAS isoform patterns differed among the graminaceous plants.

• 社団法人日本土壌肥料学会の論文

著者

• Nishizawa Naoko

  Laboratory Of Plant Biotechnology Graduate School Of Agricultural And Life Sciences The University O

• MORI Satoshi

  Laboratory of Plant Biotechnology, Graduate School of Agricultural and Life Sciences, The University

• HIGUCHI Kyoko

  Laboratory of Plant Production Chemistry, Department of Applied Biology and Chemistry, Tokyo Univers

• SUZUKI Kazuya

  Laboratory of Plant Molecular Physiology, Department of Applied Biological Chemistry, The University

• NAKANISHI Hiromi

  Laboratory of Plant Molecular Physiology, Department of Applied Biological Chemistry, The University

• Suzuki Kiyonori

  Department Of Materials Science Kitami Institute Of Technology

• Nishizawa Naoko-kishi

  Lab Plant. Mol. Physiol. Tokyo Univ. Fac. Pharmaceutical. Science. Nagoya City Univ.

• Mori Satoshi

  Laboratory Of Piant Molecular Physiology Department Of Applied Biological Chemistry The University O

• Mori Satoshi

  Lab Plant. Mol. Physiol. Tokyo Univ. Fac. Pharmaceutical. Science. Nagoya City Univ.

• Higuchi K

  Laboratory Of Plant Production Chemistry Department Of Applied Biology And Chemistry Tokyo Universit

• Nishizawa Naoko

  Environmental Science Tohoku University

• Higuchi Kyoko

  Tokyo Univ. Agriculture Tokyo Jpn

• Mori S

  Meijo Univ. Aichi Jpn

• Nakanishi Hiromi

  Laboratory Of Plant Molecular Physiology The University Of Tokyo

• Higuchi Kyoko

  Laboratory Of Plant Production Chemistry Department Of Applied Biology And Chemistry Faculty Of Appl

• Higuchi Kyoko

  Laboratory Of Plant Production Chemistry Department Of Applied Biology And Chemistry Faculty Of Applied Bio-science Tokyo University Of Agriculture

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