Expression of a Gene Specific for Iron Deficiency (Ids3) in the Roots of Hordeum vulgare

概要

論文の詳細を見る
To clone genes required for the synthesis of mugineic acid (MA) or for the transport of Fe(III)-MA, a λZAPII cDNA library was constructed from poly(A)^+-RNA isolated from Fe-deficient barley roots. The cDNA library was then used for differential screening of barley roots that had been grown in the presence and absence of iron. Seven clones that hybridized specifically, to the probe for Fe deficiency were selected. One clone, presumably encoding a full-length mRNA, as deduced from Northern hybridization, was sequenced. The clone consisting of 1685 nucleotides encoded a putative protein of 169 amino acids and an M_r of 18704. The gene was specifically expressed in the roots of iron-deficient barley. A search for homologies in a protein database (NBRF) revealed that the predicted protein product has a functional peptide domain that resembles that of 2-oxoglutarate-dependent dioxygenases.
日本植物生理学会の論文