Construction of Artificial Promoters Highly Responsive to Iron Deficiency
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概要
- 論文の詳細を見る
Iron deficiency-responsive element 1 (IDE1) and IDE2 are cis-acting elements that are responsible for Fe-deficiency-inducible and root-specific expression of the barley (Hordeum vulgare L.) gene IDS2 (Fe-deficiency-specific clone no. 2). Using these cis-acting elements, we aimed to construct super-promoters that would induce prominent gene expression in the roots of Fe-deficient rice plants (Oryza sativa L.). Modules containing IDE1 and IDE2 of the IDS2 promoter were used as repeats or were linked to the Fe-deficiency-responsive promoter of barley IDS3, and were connected to known enhancer-like sequences. Five artificial promoters, as well as the native promoters of barley IDS2 or IDS3, were connected individually upstream of β-glucuronidase (GUS) and were introduced into rice. Transgenic rice plants were grown under control or Fe-deficient conditions, and GUS expression was analyzed. The artificial promoter that contained one module of IDE1 and IDE2 conferred strong Fe-deficiency-inducible GUS expression to the roots of rice plants. Each of the five artificial promoters induced a similar level of GUS expression in Fe-deficient roots, which did not exceed the GUS expression driven by the native IDS2 or IDS3 promoter. Artificial and native promoters induced GUS expression in response to Fe-deficiency in leaves, although the level of expression was lower than that in roots. Histochemical observations revealed that GUS expression driven by artificial and native promoters was spatially similar, and expression was dominant within vascular bundles and root exodermis. These findings suggest that there is coordinated expression of the genes that are involved in Fe-deficiency-induced Fe uptake in rice.
- 社団法人日本土壌肥料学会の論文
著者
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Nishizawa Naoko
Laboratory Of Plant Biotechnology Graduate School Of Agricultural And Life Sciences The University O
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Mori S
Laboratory Of Plant Biotechnology Graduate School Of Agricultural And Life Sciences The University O
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Kobayashi Takanori
Graduate School of Agricultural and Life Sciences, The University of Tokyo
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Nakanishi Hiromi
Graduate School of Agricultural and Life Sciences, The University of Tokyo
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Nishizawa Naoko
Graduate School of Agricultural and Life Sciences, The University of Tokyo
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MORI Satoshi
Graduate School of Agricultural and Life Sciences, The University of Tokyo
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Takahashi Michiko
Graduate School of Agricultural and life Sciences, The University of Tokyo
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Nakayama Yuko
Graduate School of Agricultural and Life Sciences The University of Tokyo
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Inoue Haruhiko
Graduate School of Agricultural and Life Sciences The University of Tokyo
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Yoshihara Toshihiro
Bio-Science Department, Central Research Institute of Electric Power Industry (CRIEPI)
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Matsuhashi S
Takasaki Advanced Radiation Research Institute Japan Atomic Energy Agency
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Nishizawa Naoko
The University Of Tokyo
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Nishizawa Naoko-kishi
Lab Plant. Mol. Physiol. Tokyo Univ. Fac. Pharmaceutical. Science. Nagoya City Univ.
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Mori S
Dept. Of Appl. Biol. Chem. Univ. Tokyo
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Mori Satoshi
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Takahashi Michiko
Department Of Global Agricultural Sciences Graduate School Of Agricultural And Life Science The Univ
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Kobayashi Takanori
Univ. Tokyo Tokyo
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Mori Satoshi
Lab Plant. Mol. Physiol. Tokyo Univ. Fac. Pharmaceutical. Science. Nagoya City Univ.
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Nishizawa N‐k
Univ. Tokyo Tokyo Jpn
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Mori S
Department Of Applied Biological Chemistry The University Of Tokyo
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Yoshihara T
National Inst. Agrobiological Resources Ibaraki Jpn
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Inoue Haruhiko
Laboratory Of Plant Biotechnology Graduate School Of Agricultural And Life Sciences The University O
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Nishizawa Naoko
Environmental Science Tohoku University
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Nishizawa Naoko
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Mori S
Meijo Univ. Aichi Jpn
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Yoshihara Toshihiro
Bio-science Dep. Central Res. Inst. Electric Power Industry
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Kobayashi Takanori
東大農
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Nishizawa Naoko-kishi
Laboratory Of Plant Nutrition And Fertilizers Department Of Agricultural Chemistry The University Of
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Nakanishi Hiromi
Graduate School Of Agricultural And Life Sciences University Of Tokyo
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Takahashi Michiko
Lab Plant. Mol. Physiol. Tokyo Univ. Fac. Pharmaceutical. Science. Nagoya City Univ.
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Nishizawa Naoko-kishi
Laboratory Of Plant Molecular Physiology The University Of Tokyo
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NISHIZAWA Naoko
Graduate School of Agricultural and Life Science, University of Tokyo
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MORI Satoshi
Graduate School of Agricultural and Life Science, University of Tokyo
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NAKANISHI Hiromi
Graduate School of Agricultural and Life Science, University of Tokyo
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TAKAHASHI Michiko
Graduate School of Agricultural and Life Science, University of Tokyo
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