Highly Sensitive Quantitative Analysis of Nicotianamine Using LC/ESI-TOF-MS with an Internal Standard
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概要
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A highly sensitive quantitative method for analyzing nicotianamine (NA) by liquid chromatography/electrospray ionization time-of-flight mass spectrometry (LC/ESI-TOF-MS) is reported. Fluorenylmethoxycarbonylation of nicotianamine reduced its polarity and enabled its retention in a reversed-phase column. The adoption of Nε-nicotyllysine (NL) as an internal standard ensured reliable quantification by giving a linear calibration curve drawn between the NA/NL molar ratios of standard solutions injected and the NA/NL area ratios in mass chromatograms. The high sensitivity of this analytical method allowed us to measure the amount of NA. This analytical method has applications to all research concerning NA.
- 社団法人 日本農芸化学会の論文
著者
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Nakanishi Hiromi
Graduate School of Agricultural and Life Sciences, The University of Tokyo
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Nishizawa Naoko
Graduate School of Agricultural and Life Sciences, The University of Tokyo
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MORI Satoshi
Graduate School of Agricultural and Life Sciences, The University of Tokyo
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Takahashi Michiko
Graduate School of Agricultural and life Sciences, The University of Tokyo
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WADA Yasuaki
Graduate School of Agricultural and Life Sciences, The University of Tokyo
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Yamaguchi Isomaro
Faculty Of Agriculture University Of Tokyo
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