Metabolic Engineering of Saccharomyces cerevisiae Producing Nicotianamine : Potential for Industrial Biosynthesis of a Novel Antihypertensive Substrate
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概要
- 論文の詳細を見る
Nicotianamine (NA), a metal chelator, is ubiquitous in higher plants. In humans, NA inhibits angiotensin I-converting enzyme (ACE), and consequently reduces high blood pressure. Nicotianamine is synthesized from the trimerization of S-adenosylmethionine (SAM) by NA synthase (NAS). Here, we aimed to produce large amounts of NA fermentatively by introducing the Arabidopsis AtNAS2 gene into Saccharomyces cerevisiae strain SCY4. This strain can accumulate up to 100 times the usual amount of SAM, and this is considered desirable for overproduction of NA. Nicotianamine was produced in the engineered yeast, and the NA level increased with incubation time until the stationary phase. The maximum concentration of intracellular NA obtained was 766±33 μg/g wet weight. Successful production of NA in S. cerevisiae should pave the way for industrial production of this novel antihypertensive substrate.
- 社団法人 日本農芸化学会の論文
- 2006-06-23
著者
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Nishizawa Naoko
Laboratory Of Plant Biotechnology Graduate School Of Agricultural And Life Sciences The University O
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Mori S
Laboratory Of Plant Biotechnology Graduate School Of Agricultural And Life Sciences The University O
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Kobayashi Takanori
Graduate School of Agricultural and Life Sciences, The University of Tokyo
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Nakanishi Hiromi
Graduate School of Agricultural and Life Sciences, The University of Tokyo
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Nishizawa Naoko
Graduate School of Agricultural and Life Sciences, The University of Tokyo
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MORI Satoshi
Graduate School of Agricultural and Life Sciences, The University of Tokyo
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Takahashi Michiko
Graduate School of Agricultural and life Sciences, The University of Tokyo
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WADA Yasuaki
Graduate School of Agricultural and Life Sciences, The University of Tokyo
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Matsuhashi S
Takasaki Advanced Radiation Research Institute Japan Atomic Energy Agency
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Nishizawa Naoko
The University Of Tokyo
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Nishizawa Naoko-kishi
Lab Plant. Mol. Physiol. Tokyo Univ. Fac. Pharmaceutical. Science. Nagoya City Univ.
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Mori S
Dept. Of Appl. Biol. Chem. Univ. Tokyo
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Mori Satoshi
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Takahashi Michiko
Department Of Global Agricultural Sciences Graduate School Of Agricultural And Life Science The Univ
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Kobayashi Takanori
Univ. Tokyo Tokyo
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Mori Satoshi
Lab Plant. Mol. Physiol. Tokyo Univ. Fac. Pharmaceutical. Science. Nagoya City Univ.
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Nishizawa N‐k
Univ. Tokyo Tokyo Jpn
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Mori S
Department Of Applied Biological Chemistry The University Of Tokyo
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Nishizawa Naoko
Environmental Science Tohoku University
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Nishizawa Naoko
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Mori S
Meijo Univ. Aichi Jpn
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Kobayashi Takanori
東大農
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Wada Yasuaki
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Nishizawa Naoko-kishi
Laboratory Of Plant Nutrition And Fertilizers Department Of Agricultural Chemistry The University Of
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Nakanishi Hiromi
Graduate School Of Agricultural And Life Sciences University Of Tokyo
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Takahashi Michiko
Lab Plant. Mol. Physiol. Tokyo Univ. Fac. Pharmaceutical. Science. Nagoya City Univ.
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Nishizawa Naoko-kishi
Laboratory Of Plant Molecular Physiology The University Of Tokyo
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NISHIZAWA Naoko
Graduate School of Agricultural and Life Science, University of Tokyo
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MORI Satoshi
Graduate School of Agricultural and Life Science, University of Tokyo
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NAKANISHI Hiromi
Graduate School of Agricultural and Life Science, University of Tokyo
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TAKAHASHI Michiko
Graduate School of Agricultural and Life Science, University of Tokyo
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