Regulation of paracellular barrier function in mouse submandibular gland acini

概要

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The paracellular pathway is a dynamic and a highly regulated transport route of salivary gland acinar secretion. In this study, the relationship between paracellular permeability and cytoskeletal proteins was investigated in order to clarify the nature of the sealing mechanism of the tight junction in mouse submandibular gland acini. Male Sic: ddy mice, weighing 60-70 g were used. Tight junctions were found impermeable to microperoxidase in the control gland. However, at 3 min after carbachol administration, tight junctions become permeable. In control acini, the tight junction protein, ZO-1 was highly concentrated at the luminal site of cytoplasm in company with occludin which was also found at the tight junction region. The distribution pattern of E-cadherin coincided completely with that of both β-catenin and vinculin. Cytoplasmic aggregates of F-actin were seen and were colocalized with both ZO-1 and vinculin. In the carbachol administered gland, expression of ZO-1 was expanded, not only to the tight junctional region, but also lateral membranes. The staining pattern of E-cadherin was not jagged or discontinuous as was the pattern observed for vinculin. Results indicate that secretory stimulation leads to alteration in the architecture of the adherens junction. Thus the adherens junction may well represent an intermediate link in the signaling pathway to the tight junction via the cytoskelton.
大阪歯科学会の論文