Post-Translational Modification of βH-Crystallin of Bovine Lens with Aging (Medicina Chemistry)
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概要
- 論文の詳細を見る
This study investigates post-translational modification of proteins of bovine lens with aging (3 year old vs. 6 month old cows). After water-soluble proteins were submitted to gel and ion exchange chromatography, βH-crystallin, a subunit of β-crystallin, and modified materials were isolated. These materials were then submitted to two dimensional polyacrylamide gel electrophoresis (2D-SDS PAGE) to detect and isolate the new spots. Results for lens proteins from 3 year old animals were compared to those from 6 month old animals. All spots were digested in gel with trypsin and the molecular masses of tryptic digests were measured by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOFMS). Peptides peaks obtained from mass mapping were identified using the protein database of the MS-Fit program in the Protein prospector program of the University of California, San Francisco. We found that two post translational modifications of βH-crystallin, acetylation and phosphorylation occurred with aging.
- 社団法人日本薬学会の論文
- 2003-12-01
著者
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MORI Satoshi
Department of Applied Biological Chemistry, Division of Agriculture and Agricultural Life Sciences,
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Mori S
Shimizu Pharmaceutical Co. Ltd. Shizuoka Jpn
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Nagai Makoto
Department Of Internal Medicine Aoto Hospital Jikei University School Of Medicine
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Takeuchi N
Showa Pharmaceutical University
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KAMEI Akira
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Meijo University
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TAKEUCHI Noriko
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Meijo University
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Mori Satoshi
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Nagai Makoto
Faculty Of Pharmaceutical Sciences Hoshi University
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Kamei A
Meijo Univ. Aichi Jpn
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Kamei A
Meijo Univ. Nagoya Jpn
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Kamei Akira
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Meijo University
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Takeuchi N
Meijo Univ. Nagoya Jpn
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Nagai Makoto
Department Of Internal Medicine Aoto Hospi Tal Jikei University School Of Medicine
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Mori Satoshi
Department Of Biochemistry Faculty Of Pharmaceutical Sciences Meijo University
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Nagai Makoto
Departement Of Cardiology Jikei University School Of Medicine
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