ライ麦染色体添加系統を用いた2′-デオキシムギネ酸合成酵素および3ハイドロキシムギネ酸合成酵素の遺伝子座乗染色体ライムギ5Rの決定〔英文〕
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概要
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Using Imperial rye chromosome addition lines of wheat, we identified the rye chromosome that carries the gene for mugineic acid (MA) synthetase catalyzing synthesis of MA from 2-deoxymugineic acid (DMA) and the gene for 3-hydroxymugineic acid (HMA) synthetase catalyzing synthesis of HMA from MA. Plants of seven addition lines, Chinese Spring wheat and Imperial rye were water-cultured, and chlorosis was induced by depletion of Fe from the culture medium. The root-washing from each plant was analyzed with HPLC to detect mugineic acid-family phytosiderophores (MAs). Chinese Spring wheat secreted only DMA, while Imperial rye secreted DMA, MA and HMA. In the case of addition lines, a 5R addition line secreted DMA, MA and HMA, but the others secreted only DMA. Therefore, 5R of Imperial rye was concluded as a carrier of the two genes which encode enzymes that catalyze MA-synthesis from DMA and HMA-synthesis from MA.
- 日本遺伝学会の論文
著者
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MORI Satoshi
Department of Applied Biological Chemistry, Division of Agriculture and Agricultural Life Sciences,
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Kishi-nishizawa Naoko
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Mori Satoshi
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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FUJUGAKI Junzo
Junior College, Tokyo University of Agriculture
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KISHI-NISHIZAWA Naoko
Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
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森 敏
Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
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西沢 直子
Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
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藤垣 順三
Junior College, Tokyo University of Agriculture
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