A High Performance Liquid Chromatography-Radio-Organic Acid Analyzer for Determining the Specific Activities of Radioactive Organic Acids
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概要
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To monitor rapidly the specific activity of radioactive organic acids, a high performance liquid chromatography (HPLC)-radioanalyzer was constructed. The instrument is a linear combination of an HPLC organic acid analyzer and a radioanalyzer. Organic acids are separated through a 100 cm column packed with Diaion-CK08S (H+type) resin. The organic acids were detected at 430 nm after reaction with bromocresol purple. The effluents from the HPLC are conduced to a U-type flow-cell packed with lithium-glass scintillator, which floresces in response to β-rays. The instrument was utilized to investigate the assimilation of H^<14>CO_3^- fixation in barley roots after 10 min uptake. Malate showed the highest specific activity, followed by citrate and aspartate.
著者
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Mori Satoshi
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Mori Satoshi
Department Of Agricultural Chemistry The University Of Tokyo
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