Suppression of NO Production in Activated Macrophages in Vitro and ex Vivo by Neoandrographolide Isolated from Andrographis paniculata
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概要
- 論文の詳細を見る
In this study, we investigated the in vitro and ex vivo suppressive effects of Andrographis paniculata on nitric oxide (NO) production in mouse peritoneal macrophages elicited by bacillus Calmette-Guein (BCG) and stimulated by lipopolysaccharide (LPS). Incubation of BCG-induced macrophages with the methanol extract of A. paniculata reduced LPS stimulated NO production. The diterpene lactones andrographolide and neoandrographolide were isolated as active components from the extract. These compounds suppressed NO production in a concentration-dependent manner in the concentration range from 0.1 to 100 μ_M and their IC_<50> values were 7.9 and 35.5μ_M. Neoandrographolide also suppressed NO production by 35 and 40% when the macrophages were collected after oral administration of neoandrographolide at doses of 5 and 25 mg/kg/d and LPS stimulated NO production was examined. However, andrographolide did not reduce NO production on oral administration at the same doses. These results indicate that neoandrographolide, which inhibited NO production both in vitro and ex vivo may play an important role in the use of A. paniculata as an anti-inflammatory crude drug.
- 公益社団法人日本薬学会の論文
- 2002-09-01
著者
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YOSHIZAKI Fumihiko
Department of Pharmacognosy, Tohoku Pharmaceutical University
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Takano Fumihide
Dep. Of Pharmacognosy And Chemistry Of Natural Products Graduate School Of Natural Sci. And Technol.
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Takano Fumihide
Kanazawa Univ. Kanazawa Jpn
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Yoshizaki Fumihiko
金沢医科大学 総合内科学
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Yoshizaki F
Department Of Pharmacognosy Tohoku Pharmaceutical University
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BATKHUU Javzan
Faculty of Biology, National University of Mongolia
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FUSHIYA Shinji
Department of Kampo Pharmaceutical Sciences, Nihon Pharmaceutical University
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BATKHUU Javzan
Experimental Station for Medicinal Plant Studies, Graduate School of Pharmaceutical Sciences, Tohoku
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HATTORI Koichi
Experimental Station for Medicinal Plant Studies, Graduate School of Pharmaceutical Sciences, Tohoku
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TAKANO Fumihide
Experimental Station for Medicinal Plant Studies, Graduate School of Pharmaceutical Sciences, Tohoku
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FUSHIYA Shinji
Experimental Station for Medicinal Plant Studies, Graduate School of Pharmaceutical Sciences, Tohoku
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OSHIMAN Ko-ichi
Tsukuba Research Institute, Sumitomo Forestry Co., Ltd.
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FUJIMIYA Yoshiaki
Tsukuba Research Institute, Sumitomo Forestry Co., Ltd.
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Oshiman Ko-ichi
Tsukuba Research Institute Sumitomo Forestry Co. Ltd.
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Hattori Koichi
Experimental Station For Medicinal Plant Studies Graduate School Of Pharmaceutical Sciences Tohoku U
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Fushiya S
Department Of Kampo Pharmaceutical Sciences Nihon Pharmaceutical University
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Yoshizaki Fumihiko
Department Of Pharmacognosy Tohoku Pharmaceutical University
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Ohba K
Tsukuba Research Institute Sumitomo Forestry Co. Ltd.
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Takano Fumihide
Department Of Pharmacognosy And Chemistry Of Natural Products Graduate School Of Natural Science And
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