Thiamine requirement of two different cultured cell lines of soybean
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概要
- 論文の詳細を見る
When supplied with 6-benzyladenine (0.5-5 mg/liter) instead of thiamine, thiamine-requiring soybean cells (strain TU) could grow successively. The effect of cytokinin was much more remarkable at a relatively higher concentration of 2,4-dichlorophenoxyacetic acid (4 mg/liter) than at a lower concentration (0.5 mg/liter) . Among calli initiated from soybean hypocotyls on a medium without thiamine, the thiamine-nonrequiring variant (strain G) was obtained incidentally. As this cell line became green in light, it could be visually separated from the other necrotic tissues. Strain G cells could grow successively not only without thiamine but also without phytohormones, auxin and cytokinin. This cell line had relatively higher amounts of chlorophyll and thiamine, and grew in rigid, large cell aggregates which differed from cell aggregates of the strain TU cell line. The thiamine requirement of plant cultured cells seems to be associated with the degree of dedifferentiation of the cells rather than the kind of plant. In general, the higher the degree of redifferentiation of the cells, the higher is their thiamine level and the less they require externally supplied thiamine.
- 日本植物生理学会の論文
著者
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Ohira Koji
Department Of Agricultural Chemistry Tohoku University
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Ikeda Makoto
Department Of Agricultural Chemistry Tohoku University:(present)section Of Phytochemical Research Ei
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Ojima Kunihiko
Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University
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Ojima Kunihiko
Department Of Agricultural Chemistry Tohoku University
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Ojima Kunihiko
Department Of Agricultural Chemistiry Faculty Of Agriculture Tohoku University
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Ohira Koji
Department Of Agricultural Cheimistry Faculty Of Agriculture Tohoku University
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Ohira Koji
Department of Agricultural Chemistry, Tohoku University
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