PROTEINASE ACTIVITIES AND LOSS OF NITROGEN IN THE SENESCING LEAVES OF FIELD-GROWN RICE (Oryza sativa L.)
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概要
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Changes in the proteinase activities for the degradation of casein and ribulose-1,5-bisphosphate carboxylase (RuBPCase) were examined in the crude extracts from the 11th and 13th leaf blades of rice (Oryza sativa L.) throughout the life span of the leaves. Nitrogen contents of the samples of both leaves decreased rapidly in the early and middle stages, and gradually in the last stage of senescence. The caseolytic activity of the 11th leaf increased once in the early stage, and then decreased rapidly in the middle stage of senescence, while the caseolytic and RuBPCase-degrading activities of the 13th leaf did not increase in the early stage of senescence. The proteinase activities remarkably increased in both leaves in the last stage of senescence, although nitrogen contents of the leaves had reached low levels and most of the remobilizable nitrogen had been already removed from the leaves. These results indicate that the in vitro proteinase activity is not necessarily correlated with the in vivo loss of nitrogen from the leaves during leaf senescence in rice plants. The pH dependence of RuBPCase-degrading activity varied with the leaf age. The highest activity was found at pH 5.5-6.0 in the mature leaves, but at pH 8.0 in the leaves at the last stage of senescence. The caseolytic activity in the extract from the mature leaves was strongly inhibited by ethylenediaminetetraacetic acid or ο-phenanthroline, while the activity in the extract from the senescing ones was inhibited by phenylmethylsulfonyl fluoride or diisopropyl fluorophosphate. The elution profiles of proteinase on a column of DEAE-cellulose showed that the activity could be separated into several or more peaks in all the extracts from the leaves at different leaf ages and the proportion of the activity of each peak varied with the leaf age. These results indicate that rice leaves contain multiple proteinases whose constituents and amounts change with the leaf age.
- 社団法人日本土壌肥料学会の論文
著者
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Ohira Koji
Department Of Agricultural Chemistry Tohoku University
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Ohira Koji
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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Mae Tadahiko
Department of Agricultural Chemistry, Tohoku University
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Mae Tadahiko
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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HOSHINO Takashi
Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University
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Ohira Koji
Department Of Agricultural Cheimistry Faculty Of Agriculture Tohoku University
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Hoshino Takashi
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University:(present Address)niiga
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Mae Tadahiko
Department Of Agricultural Chemistry Faculty Of Agriculture
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