Thiamine requirements of various plant cells in suspension culture
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概要
- 論文の詳細を見る
The thiamine requirements of cells of several plant species in suspension culture were investigated. Omission of thiamine from the medium leads to a rapid and complete cessation of growth in subcultures of soybean, tobacco and rice cells. The critical levels of thiamine content in these cells are considered to be in the vicinity of 0.6, 0.5 and 0.2 μg per g dry weight, respectively. Soybean cells can grow satisfactorily when supplied with an equimolar mixture of two thiamine precursors, pyrimidine and thiazole moieties. Neither moiety alone can substitute for thiamine. On the other hand, Ruta and peanut cells can be successfully subcultured for ten passages in the absence of externally supplied thiamine. When grown without thiamine, Ruta cells had all average thiamine content of 0.5-0.6 μg in darkness and 3.5 μg per g dry weight in the light. The relationship between the thiamine requirement and the degree of dedifferentiation cultured cells is discussed.
- 日本植物生理学会の論文
著者
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Ohira Koji
Department Of Agricultural Chemistry Tohoku University
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Ikeda Makoto
Department Of Agricultural Chemistry Tohoku University:(present)section Of Phytochemical Research Ei
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Ojima Kunihiko
Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University
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Ojima Kunihiko
Department Of Agricultural Chemistry Tohoku University
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Ojima Kunihiko
Department Of Agricultural Chemistiry Faculty Of Agriculture Tohoku University
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Ohira Koji
Department Of Agricultural Cheimistry Faculty Of Agriculture Tohoku University
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Ohira Koji
Department of Agricultural Chemistry, Tohoku University
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