Studies on the nutrition of rice cell culture I. A simple, defined medium for rapid growth in suspension culture
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概要
- 論文の詳細を見る
We investigated the nutrient requirements of rice in liquid culture and developed a revised medium of mineral salts, sucrose, thiamine and 2,4-dichlorophenoxyacetic acid. The following major nutrients at the indicated concentrations were beneficial : NO_3-N (40 mM), NH_4-N (5.0 mM), P (2.0 mM) and K (40 mM). Cobalt, iodine, pyridoxine, nic.otinic acid and m-inositol were not essential and were excluded from the revised R-2 medium. Growth was better with ammonium and nitrate together than with nitrate as the sole nitrogen source. Cell growth in the R-2 medium was superior to that obtained in B5, Heller, Murashige-Skoog and White media.
- 日本植物生理学会の論文
著者
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Fujiwara Akio
Department of Clinical Pharmacology, Medical College of Oita
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Fujiwara Akio
Department Of Agricultural Ohemistry Faculty Of Agriculture Tohoku University
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Ohira Koji
Department Of Agricultural Chemistry Tohoku University
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Ohira Koji
Department Of Agricultural Ohemistry Faculty Of Agriculture Tohoku University
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Ohira Koji
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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Ojima Kunihiko
Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University
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Ojima Kunihiko
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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Ojima Kunihiko
Department Of Agricultural Chemistiry Faculty Of Agriculture Tohoku University
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Fujiwara Akio
Department Nf Agricultural Chemistry Faculty Of Agriculture Tohoku University
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Ohira Koji
Department Of Agricultural Cheimistry Faculty Of Agriculture Tohoku University
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