The Large Subunit of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase is Fragmented into 37-kDa and 16-kDa Polypeptides by Active Oxygen in the Lysates of Chloroplasts from Primary Leaves of Wheat
スポンサーリンク
概要
- 論文の詳細を見る
Lysates of chloroplasts isolated from wheat (Triticum aestivum L. cv. Aoba) leaves were incubated on ice (pH 5.7) for 0 to 60 min in light (15 μmol quanta m^<-2> s^<-1>), and degradation of the large subunit (LSU) of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco: EC 4.1.1.39) was analyzed by applying immunoblotting with site-specific antibodies against the N-terminal, internal, and C-terminal amino acid sequences of the LSU of wheat Rubisco. The most dominant product of the breakdown of the LSU and that which was first to appear was an apparent molecular mass of 37-kDa fragment containing the N-terminal region of the LSU. A 16-kDa fragment containing the C-terminal region of the LSU was concomitantly seen. This fragnrentation of the LSU was inhibited in the presence of EDTA or 1,10-phenanthroline. The addition of active oxygen scavengers, catalase (for H_2O_2) and n-propyl gallate (for hydroxyl radical) to the lysates also inhibited the fragmentation. When the purified Rubisco from wheat leaves was exposed to a hydroxyl radical-generating system comprising H_2O_2, FeSO_4 and ascorbic acid, the LSU was degraded in the same manner as observed in the chloroplast lysates. The results suggest that the large subunit of Rubisco was directly degraded to the 37-kDa fragment containing the N-terminal region and the 16-kDa fragment containing the C-terminal region of the LSU by active oxygen, probably the hydroxyl radical, generated in the lysates of chloroplasts.
- 日本植物生理学会の論文
著者
-
ISHIDA Hiroyuki
Department of Pediatrics, Matsushita Memorial Hospital
-
Ishida Hiroyuki
Graduate School Of Agricultural Science Tohoku University
-
Mae Tadahiko
Department of Agricultural Chemistry, Tohoku University
-
Mae Tadahiko
Tohoku Univ. Sendai Jpn
-
Mae Tadahiko
Grad. Schl. Agr. Sci. Tohoku Univ.
-
Mae Tadahiko
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
-
MAKINO Amane
Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University
-
Nishimori Yoshito
Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University
-
Sugisawa Miki
Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University
-
Mae Tadahiko
Dept. Of Appl. Biol. Chem. Fac Of Agric. Tohoku Univ.
-
Sugisawa Miki
Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University
-
Nishimori Yoshito
Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University
-
Ishida Hiroyuki
Institute For Chemical Research Kyoto University
-
Ishida H
Keio Univ. School Of Medicine
-
Ishida Hiroyuki
Department Of Chemistry Okayama University
-
Ishida Hiroyuki
Department Of Chemistry College Of General Education Okayama University
-
Mae Tadahiko
Department Of Agricultural Chemistry Faculty Of Agriculture
-
MAKINO Amane
Department of Agriculture, Graduate School of Agricultural Science, Tohoku University:CREST, JST
-
Ishida Hiroyuki
Department of Applied Plant Science, Graduate School of Agricultural Science, Tohoku University
-
MAKINO Amane
Department of Agriculture, Graduate School of Agricultural Science, Tohoku University
関連論文
- PLASMA ANGIOTENSIN II LEVELS IN ESSENTIAL AND SECONDARY HYPERTENSIVES : PROCEEDINGS OF THE 47th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY : Hypertension
- Outcome of Non-T-Cell-Depleted HLA-Haploidentical Hematopoietic Stem Cell Transplantation from Family Donors in Children and Adolescents
- Synthesis, Structures, and Magnetic Properties of Doubly Face-Sharing Heterotrinuclear Ni^-Ln^-Ni^ (Ln = Eu, Gd, Tb, and Dy) Complexes
- Fractionation of Nitrogen Isotopes by Glutamine Synthetase Isolated from Spinach Leaves
- Metabolism of S-methylcysteine and pectin esterification in Chinese cabbage, Brassica pekinensis Rupr.
- Metabolism of S-methylcysteine and its sulfoxide in Chinese cabbage, Brassica pekinensis Rupr.
- Fate of (+)S-methyl-L-cysteine sulfoxide in chinese cabbage, Brassica pekinensis RUPR.
- Pore Structure of Hard Carbon Made from Phenolic Resin Studied by ^Xe NMR
- REGULATION OF RUBISCO ACTIVITY BY LIGHT INTENSITY AND ELEVATED CO_2 LEVELS IN TRANSGENIC RICE WITH DECREASED RUBISCO
- PHOTOPROTECTION IN TRANSGENIC RICE PLANTS WITH DECREASED RUBISCO
- Control of hepatic veno-occlusive disease with an antithrombin-III concentrate-based therapy
- Granulocytic Sarcoma Presenting with Severe Adenopathy (Cervical Lymph Nodes, Tonsils, and Adenoids) in a Child with Juvenile Myelomonocytic Leukemia and Successful Treatment with Allogeneic Bone Marrow Transplantation
- Streptococcus pneumoniae-associated arthritis of a lumbar facet joint in a 4-year-old boy
- Hydrogen Transfer in a Hydrogen-Bonded 1:2 Complex of Chloranilic Acid with 1,2-Diazine Studied by ^CI NQR
- ^1H, ^2H, ^F, ^P, and ^ClNMR Studies on Molecular Motions in Ionic Plastic Phases of Pyrrolidinium Perchlorate and Hexafluorophosphate
- Molecular Motions in Highly Disordered Solid Phases of [(CH_3)_3NCH_2CH_3]X (X=ClO_4, PF_6, NO_3)Studied by ^lH, ^F, and ^N NMR, Powder X-Ray Diffraction, and Differential Scanning Calorimetry
- Preparation and Extraction of Ca@C_
- Effects of Sulfur Nutrition on the Growth and Photosynthesis of Rice
- DOES DECREASE IN RUBISCO BY 'ANTISENSE' rbcS LEAD TO A HIGHER N-USE EFFICIENCY OF PHOTOSYNTHESIS AT ELEVATED CO_2 IN RICE PLANTS?
- INHIBITION OF PHOTOSYNTHESIS BY POD REMOVALS IS NOT CAUSED BY DECREASES IN THE PHOTOSYNTHETIC PROTEINS IN BEAN LEAVES
- EFFECTS OF IRRADIANCE ON THE RATE OF PLANT GROWTH AND PHOTOSYNTHESIS IN RICE
- EFFECTS OF HIGH CO_2 ON THE PHOTOSYNTHETIC CHARACTERISTICS IN RICE LEAVES
- Effects of Panicle Removal on the Photosynthetic Characteristics of the Flag Leaf of Rice Plants during the Ripening Stage : ENVIRONMENTAL AND STRESS RESPONSES : PROTEINS, ENZYMES AND METABOLISM
- Enrichment of Ce@C_ by HPLC Technique
- Effect of Light Intensity on the Growth and Photosynthesis of Rice under Different Sulfur Concentrations
- In Situ Observation of Photon-Stimulated Hydrogen Removal on a HF-Passivated Si(111) Surface by Ultraviolet Photoelectron Spectroscopy Using Synchrotron Radiation
- Low-Temperature Cleaning of HF-Passivated Si(111) Surface with VUV Light
- A Very Sensitive Direct Radioimmunoassay System for Plasma Angiotensin II and its Clinical Application in Various Hypertensive Diseases : THE 13TH CONFERENCE ON THE PATHOGENESIS OF HYPERTENSION
- In vivo Fragmentation of the Large Subunit of Ribulose-1,5-Bisphosphate Carboxylase by Reactive Oxygen Species in an Intact Leaf of Cucumber under Chilling-light Conditions
- Exclusion of Ribulose-1,5-bisphosphate Carboxylase/oxygenase from Chloroplasts by Specific Bodies in Naturally Senescing Leaves of Wheat
- The Degradation of the Large Subunit of Ribulose-1,5-bisphosphate Carboxylase/oxygenase into the 44-kDa Fragment in the Lysates of Chloroplasts Incubated in Darkness
- DEGRADATION OF STROMAL PROTEINS IN ISOLATED CHLOROPLASTS : MECHANISMS OF FRAGMENTATION OF GLUTAMINE SYNTHETASE MEDIATED BY REACTIVE OXYGEN SPECIES
- DEGRADATION OF STROMAL PROTEINS IN ISOLATED CHLOROPLASTS : MECHANISMS OF RUBISCO AND GS FRAGMENTATION MEDIATED BY REACTIVE OXYGEN SPECIES
- DEGRADATION OF GLUTAMINE SYNTHETASE MEDIATED BY REACTIVE OXYGEN SPECIES IN ILLUMINATED CHLOROPLASTS
- Rubisco: Photosynthesis and its protein turnover
- CABP INHIBITS THE FRAGMENTATION OF THE RUBISCO-LSU BY ACTIVE OXYGEN
- The Large Subunit of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase is Fragmented into 37-kDa and 16-kDa Polypeptides by Active Oxygen in the Lysates of Chloroplasts from Primary Leaves of Wheat
- FRAGMENTATION OF THE LARGE SUBUNIT OF RUBISCO BY ACTIVE OXYGEN IN WHEAT CHLOROPLASTS UNDER ILLUMINATION
- CHARACTERISTICS OF RUBISCO DEGRADATION IN THE LYSATES OF ISOLATED CHLOROPLASTS FROM WHEAT LEAVES
- PROTEINASES IN SENESCING MAIZE (Zea mays L.) LEAVES
- PROTEINASE ACTIVITIES AND LOSS OF NITROGEN IN THE SENESCING LEAVES OF FIELD-GROWN RICE (Oryza sativa L.)
- Relation between Leaf Age and Nitrogen Incorporation in the Leaf of the Rice Plant (Oryza sativa L.)
- PHOTOSYNTHETIC CHARACTERISTICS IN RICE LEAVES GROWN UNDER THE CONDITIONS OF PHYSIOLOGICALLY LOW TEMPERATURE WITH HIGH IRRADIANCE
- Gas Exchange Characteristics in Rice Leaves Grown under the Conditions of Physiologically Low Temperature and Irradiance
- EFFECTS OF LONG-TERM WATER STRESS ON PHOTOSYNTHESIS OF RICE LEAVES
- Photosynthesis and Plant Growth at Elevated Levels of CO_2
- Changes in the Thermal Dissipation and the Electron Flow in the Water-Water Cycle in Rice Grown Under Conditions of Physiologically Low Temperature
- Xanthophyll Cycle Pigments and Water-Water Cycle in Transgenic Rice with Decreased Amounts of Ribulose-1,5-Bisphosphate Carboxylase and the Wild-Type Rice Grown under Different N Levels (Plant Nutrition)
- PHOTOPROTECTION MECHANISMS IN RUBISCO-ANTISENSE RICE PLANTS
- WHOLE-PLANT GROWTH IN TRANSGNIC RICE PLANTS WITH DECREASED RUBISCO UNDER DIFFERENT CO_2 PARTIAL PRESSURES
- PHOTOPROTECTION OF RUBISCO-ANTISENSE RICE PLANTS OBSERVED WITH AN OPEN GAS-EXCHANGE SYSTEM EQUIPPED WITH A CHLOROPHYLL FLUOROMETER
- Leaf Development in Lolium temulentum : Photosynthesis and Photosynthetic Proteins in Leaves Senescing under Different Irradiances
- Variations in the Contents and Kinetic Properties of Ribulose-1,5-bisphosphate Carboxylases among Rice Species
- Colorimetric Measurement of Protein Stained with Coomassie Brilliant Blue R on Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis by Eluting with Formamide
- Changes in Photosynthetic Capacity in Rice Leaves from Emergence through Senescence : Analysis from Ribulose-1,5-bisphosphate Carboxylase and Leaf Conductance
- Relation between Nitrogen and Ribulose-1,5-bisphosphate Carboxylase in Rice Leaves from Emergence through Senescence
- Relation between Ribulose Bisphosphate Carboxylase Content and Chloroplast Number in Naturally Senescing Primary Leaves of Wheat
- EFFECT OF NITROGEN, PHOSPHORUS OR POTASSIUM ON THE PHOTOSYNTHETIC RATE AND RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE CONTENT IN RICE LEAVES DURING EXPANSION
- Purification and Storage of Ribulose 1,5-bisphosphate Carboxylase from Rice Leaves
- Changes in the Amounts of Ribulose Bisphosphate Carboxylase Synthesized and Degraded during the Life Span of Rice Leaf (Oryza sativa L.)
- Changes in Rubulose-1, 5-Bisphosphate Carboxylase/Oxygenase Turnover is the Key to Photosynthetic Acclimation to Elevated CO_2 in Rice(Frontiers in Rice Science -from Gene to Field-,The 100^ Anniversary of Tohoku University, International Symposium)
- Amount of Ribulose-1, 5-bisphosphate Carboxylase/Oxygenase (Rubisco) Protein and Levels of mRNAs of rbcS and rbcL in the Leaves at Different Positions in Transgenic Rice Plants with Decreased Content of Rubisco(Plant Nutrition)
- Analysis of factors for changes in the amount of ribulose-1,5-bisphosphate carboxylase/oxygenase in rice leaves from emergence through senescence
- Improvement of Physiological N-Use Efficiency in Rice Plants(Frontiers in Rice Science -from Gene to Field-,The 100^ Anniversary of Tohoku University, International Symposium)
- Mechanisms of methyl-carbon transfer from S-methylcysteine to pectin in Chinese cabbage (Brassica pekinensis Rupr.)
- Differences between Maize and Rice in N-use Efficiency for Photosynthesis and Protein Allocation
- DIFFERENCES BETWEEN RICE AND WHEAT IN RuBP REGENERATION CAPACITY PER UNIT OF LEAF NITROGEN
- H-Transfer Motions in an H-Bonded (Chloranilic Acid)-(1, 3-Diazine) 1:2 Molecular Complex Studied by ^ClNQR and ^1H NMR
- Measurement of Impact Load by using an Inverse Analysis Technique : Comparison of Methods for Estimating the Transfer Function and its Application to the Instrumented Charpy Impact Test
- Increased Rubisco Content in Transgenic Rice Transformed with the 'Sense' rbcS Gene
- Generation of Training Data by Degradation Models for Traffic Sign Symbol Recognition(Image Recognition and Understanding)
- Effect of Sodium Dodecylsulfate on the Degradation of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase in the Extract of Rice (Oryza sativa L.) Leaves
- THE RELATIONSHIP BETWEEN PROTEOLYTIC ACTIVITY AND LOSS OF SOLUBLE PROTEIN IN RICE LEAVES FROM ANTHESIS THROUGH SENESCENCE
- Changes in the Levels of Chlorophyll and Light-Harvesting Chlorophyll a/b Protein of PS II in Rice Leaves Aged under Different Irradiances from Full Expansion through Senescence : ENVIRONMENTAL AND STRESS RESPONSES : PROTEINS, ENZYMES AND METABOLISM
- Nitrogen Accumulation in the Inferior Spikelet of Rice Ear during Ripening
- Degradation of Ribulose-1,5-bisphosphate Carboxylase/Oxygenase in the Lysates of the Chloroplasts Isojated Mechanically from Wheat (Triticum aestivum L.) Leaves
- Solid-State ^C NMR Analysis of the Crystalline-Noncrystalline Structure and Chain Conformation of Thermotropic Liquid Crystalline Polyester
- Solid-State ^CNMR Studies of the Structure and Chain Conformation of Thermotropic Liquid Crystalline Polyether Crystallized from the Liquid Crystalline Glassy Phase
- Formation of the Liquid Crystalline Glassy Phase and Cold Crystallization of a New Crystal Form from the Glassy Phase for Thermotropic Liquid Crystalline Polyether
- Molecular Dynamics Simulations of a Main-Chain Liquid Crystalline Polyether in the Crystalline State : 1. Chain Conformation and Dynamics of the Spacer Methylene Sequences
- Molecular Rearrangement Accompanied by Solid-State Isomerization of {N,N'-Di-3-ethoxysalicylidene-(R,S) (S,R)-1,2-diphenyl-1,2-ethanediamine} oxovanadium(IV)
- Experimental Result on Reducing the Resistance Due To Temperature Rise in Pd Contacts
- Shift of the Major Sink from the Culm to the Panicle at the Early Stage of Grain Filling in Rice (Oryza sativa L. cv. Sasanishiki)
- Rubisco and Nitrogen Relationships in Rice : Leaf Photosynthesis and Plant Growth
- The Remobilization of Nitrogen Related to Leaf Growth and Senescence in Rice Plants (Oryza sativa L.)
- Effects of Physical Training and Mental Practice of In-clothes Swimming : Assessment by Physiological Parameters
- Phase Transitions and Ionic Motions Including Self-Diffusion in Solid Trimethylammonium Tetrafluoroborate Studied by ^1H and ^F NMR
- Comparative Research on Environmental Education between Thailand and Japan
- An Ionic Plastic Phase of 1,1-Dimethylhydrazinium Tetrafluoroborate Revealed by ^1H and ^F NMR and Thermal Measurements
- ^1H and ^F NMR Studies on Phase Transitions and Ionic Motions in Choline Tetrafluoroborate
- Arrangement and Dispersion of Rh and Pt Atoms on Graphene Oxide Sheets
- O_2-enhanced induction of photosynthesis in rice leaves : the Mehler-ascorbate peroxidase (MAP) pathway drives cyclic electron flow within PSII and cyclic electron flow around PSI(Plant nutrition)
- O_2 supports 3-phosphoglycerate-dependent O_2 evolution in chloroplasts from spinach leaves(Plant nutrition)
- Role of Rare Earth Ions (Gd, Dy, Ho and Er) in Phase Formation and Superconducting Properties of Ba1-xLnxCuO3-y Compounds
- Functional Analysis of the AKR4C Subfamily of Arabidopsis thaliana: Model Structures, Substrate Specificity, Acrolein Toxicity, and Responses to Light and [CO2]
- Scavenging Systems for Reactive Carbonyls in the Cyanobacterium Synechocystis sp. PCC 6803
- Molecular Motions of t-Butylammonium Ions and Structural Phase Transitions in Solid t-Butylammonium Perchlorate Studied by 1H NMR and Thermal Measurements.
- Measurement of the Constriction Resistance in Palladium Contacts Heated by a Heater (機構デバイス)
- 1H NMR studies on the motion of cations in the three solid phases of methylammonium perchlorate including cationic self-diffusion in its highest-temperature solid phase.
- Motion of methylammonium ions in solid CH3NH3NO3 and CH3NH3SCN studied by 1H NMR.