A Very Sensitive Direct Radioimmunoassay System for Plasma Angiotensin II and its Clinical Application in Various Hypertensive Diseases : THE 13TH CONFERENCE ON THE PATHOGENESIS OF HYPERTENSION
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概要
- 論文の詳細を見る
A very sensitive and simplified direct radioimmunoassay system for plasma angiotensin II was developed using the antiserum against synthetic angiotensin II (final dilution = 1:1,500,000) in combination with ^<125>I-labeled angiotensin II (specific activity = 1,600μCi/μg). In this assay system, it was possible to carry out a direct assay using 100μl of plasma without any extraction procedure. This conclusion was supported by 100% recovery, parallelism of plasma samples against the standard curve, and no difference in hormone levels, there was also a high positive correlation between the plasma angiotensin II levels measured by this direct assay and the dowex column extraction method. The sensitivity of this assay system was 0.1 pg/tube, which is the highest sensitivity in studies reported to date. The cross-reactivities of angiotensin III and I against this antiserum were 100% and less than 0.1%, respectively, suggesting that the antiserum was very specific for the C-terminal of angiotensin II. Plasma angiotensin II levels in normal subjects after overnight fasting ranged from 3.0 to 21.3 pg/ml (12.0±2.1 pg/ml, mean ±SE). By comparison, plasma angiotensin Ii levels of patients with essentiel hypertension were similar to those in the normal renin group, lower than those in low renin group and higher than those in high renin group. In patients with secondary hypertension, levels were lower in those with primary aldosteronism and higher in those with renovascular hypertension when compared to normal subjects.
- 社団法人日本循環器学会の論文
- 1984-11-20
著者
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Shimamoto Kazuaki
手稲渓仁会病院 総合内科
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Takahashi Nobuki
Department Of Clinical Studies And Genetics Radiation Effects Research Foundation
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IIMURA Osamu
Division of Nephrology, Department of Internal Medicine, Jichi Medical School
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Iimura Osamu
The Second Department Of Internal Medicine Sapporo Medical College
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SHIMAMOTO KAZUAKI
The Second Department of Internal Medicine, Sapporo Medical College
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Nakahashi Yasuyuki
The Second Department of Internal Medicine, Sapporo Medical College
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Hosoda Susumu
The Second Department of Internal Medicine, Sapporo Medical College
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Nishitani Takahiro
The Second Department of Internal Medicine, Sapporo Medical College
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Ishida Hiroyuki
The Second Department of Internal Medicine, Sapporo Medical College
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IIMURA Osamu
Hokkaido JR Sapporo Hospital
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Ishida Hiroyuki
Graduate School Of Agricultural Science Tohoku University
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Iimura O
Sapporo Medical Univ.
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Iimura O
Sapporo Medical University
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Iimura Osamu
Department Of Nephrology Jichi Medical School
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Iimura Osamu
The 2nd Department Of Internal Medicine Sapporo Medical College. Sapporo. Japan
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Iimura Osamu
The 2nd Department Of Internal Medicine Sapporo Medical College
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Nishitani Takahiro
The Second Department Of Internal Medicine Sapporo Medical College
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Iimura Osamu
The 2nd. Department Of Internal Medicine Sapporo Medical College
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Iimura Osamu
The Second Department Of Internal Medicine Sappolo Medical College
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TANAKA SHIGEMICHI
The Second Department of Internal Medicine,Sapporo Medical College
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Shimamoto Kazuaki
The 2nd Department Of Internal Medicine Sapporo Medical University School Of Medicine
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YOKOYAMA TOYOJI
The Second Department of Internal Medicine, Sapporo Medical College
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Yokoyama Toyoji
The Second Department Of Internal Medicine Sapporo Medical College
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Tanaka Shigemichi
The Second Department Of Internal Medicine Sapporo Medical College
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Nakahashi Yasuyuki
The Second Department Of Internal Medicine Sapporo Medical College
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Shimamoto Kazuaki
The Seceond Department Of Internal Medicine Sapporo Medical University School Of Medicine
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Hosoda Susumu
The Second Department Of Internal Medicine Sapporo Medical College
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Takahashi Nobuyuki
Radiation Effects Research Foundation Hiroshima
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Ishida Hiroyuki
Institute For Chemical Research Kyoto University
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Ishida H
Keio Univ. School Of Medicine
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Iimura O
Department Of Nephrology Jichi Medical School
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Iimura Osamu
The Secone Department Of Internal Medicine Sapporo Medical College
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Iimura Osamu
The 2nd Department Of Internal Medieine Sapporo Medical College Sapporo 060
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IIMURA OSAMU
The Second Department of Internal Mdicine, Sapporo Medical College
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NAKAHASHI YASUYUKI
The Second Department of Internal Mdicine, Sapporo Medical College
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ISHIDA HIROYUKI
The Second Department of Internal Mdicine, Sapporo Medical College
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TANAKA SHIGEMICHI
The Second Department of Internal Mdicine, Sapporo Medical College
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NISHITANI TAKAHIRO
The Second Department of Internal Mdicine, Sapporo Medical College
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HOSODA SUSUMU
The Second Department of Internal Mdicine, Sapporo Medical College
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