ラット腹水肝癌の寒天平板拡散法における制ガン物質による阻止円形成

概要

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An agar diffusion technique was applied for assaying antitumor agents by using four strains of rat ascites hepatoma (AH 7974,AH 66F, AH 13R, AH 13C) and cultured FM3A cells. The agar diffusion plate consisted of two layers ; a base layer of 1% agar medium and a seed layer containing 0.6% agar and a certain number of tumor cells. The number of cells inoculated was 2.0×10^6 cells in the case of rat ascites hepatoma. The paper discs were placed 3-5 hours after inoculation, and kept unremoved until observation. All of the antitumor substances tested (nitrogen mustard N-oxide, thio-TEPA, S261,Mitomycin-C, Chromomycin-A_3,6-mercaptopurine) inhibited growth of cells and produced inhibition zone of various sizes around the paper disc. The activity of 2-amino-6-mercaptopurine was different from them. Its inhibition zone was not visible, but was revealed as a blue inhibitory zone by using indicator dye of 2,6-dichlorophenol indophenol. When a large number of cells was inoculated, the size of inhibition zone decreased. The correlation between the size of inhibition zone and the concentration of substances was linear. Comparison of the present method with some reported procedures was also discussed.
社団法人日本薬学会の論文
1969-06-25