牛膝の成分研究(第6報) : 昆虫変態ホルモンの定量

概要

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Chemical method for the determination of ecdysterone (I) and inokosterone (II) was examined. Both I and II show ultraviolet absorption with a maximum at 250 mμ and the use of this wave length for their determination was attempted. Since this absorption maximum disappeared on the application of sodium borohydride, I and II can be determined from the decrease of this absorption. Application of sulfuric acid to I and II produces fluorescence and the fluorescence intensity of II increases markedly when made ammonia alkaline. Utilization of this fact made it possible to determine II in a mixture of I and II. Linearity was established between the mixture of I and II, and a difference in the fluorescence intensity in sulfuric acidity and ammoniac alkalinity, and this fact was utilized to determine the mixing ratio of I and II in their mixture.
1968-01-25