胃腸薬に配合されている消化酵素の簡易活性測定法(第1報)蛋白質消化酵素活性

概要

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Proteolytic enzymes are contained in many digestive and gastrointestinal drugs. The proteolytic activities are commonly determined by casein-folin method. Since this method requires complicated procedure and long time, it was attempted to develop a simple method of determining proteolytic enzymes in gastrointestinal drugs for the routine quality control of the drugs. Azocoll used as substrate is a collagen combining with an azo dye to provide an insoluble substrate. Proteolytic enzymes digest the dispersed substrate and release colored hydrolyzed products to the solution. The reaction is simply terminated by filtering off the substrate. The activities can be given by determining the absorbance of the filtrate as an index. When this method was applied to the determination of the activity of proteolytic enzymes contained in the gastrointestinal drugs, the following several advantages were found compaired with the casein-folin method ; i) simple and quick procedure, ii) high sensitivity, iii) long time stability of the colored hydrolyzed products, iv) good correlation with the conventinal method.
1980-09-25