ゲンノショウコの有効成分の研究(第2報) : 腸管収縮活性物質のAcetylcholinesteraseによる加水分解

概要

論文の詳細を見る
Hydrolysis of the fraction with intestinal contractile activity from Geranii Herba (G-A) by acetylcholinesterase (Ach-esterase) was investigated. G-A was more stable than acetylcholine (Ach) to the hydrolysis by Ach-esterase, this stability being apparently due to the coexistence of choline (Ch) which was a competitive inhibitor for Ach-esterase. The Ach-like substance contained in G-A was considered to be Ach itself: when purified by means of preparative paper partition chromatography the substance was easily hydrolysed by Ach-eseterase. The ratio of Ach to Ch in G-A was estimated to be 1:1,000-2,000 from the rate of hydrolysis of the mixture of Ach and Ch by Ach-esterase. As Ach is stable in weak acidic medium, the pH of the medium along with the presence of a competitive inhibitor, Ch, seems to contribute to the stability of Ach in the extract of Geranii Herba, i.e. G-A.
日本生薬学会の論文
1980-06-20