イオン会合性試薬による塩酸ケタミンおよび塩酸キニーネの吸光光度定量

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Ketamine・HCl or quinine・HCl forms an ion-association complex with ion association reagents such as tropaeoline 00 and erio green B, and the complex can be extracted into dichloromethane. The concentration of ketamine・HCl or quinine HCl in aqueous solution is determined by measuring the absorbance of extracts at 543 nm (ketamine・HCl) or 637 nm (quinine・HCl). Calibration curve obeyed to Beer's law in the range of 1.82×10^<-8> to 2.55×10^<-7> mol/ml (ketamine・HCl) or 2.52×10^<-9> to 6.30×10^<-8> mol/ml (quinine・HCl), respectively. The determination procedure is as follows : 1 ml of sample solution, 1 ml of 2.72×10^<-6> mol/ml (tropaeoline 00) or 2.28×10^<-6> mol/ml (erio green B) reagent, 2 ml of Britton-Robinson buffer solution, and 5 ml of dichloromethane are placed in a 15 ml of test tube. A mixture is shaken for 3 min mechanically and allowed to stand for 30 min. Absorbance of the dichloromethane layer is measured at 637 nm for the determination of quinine・HCl. For ketamine HCl, to 2 ml of dichloromethane layer, 0.5 ml of 10% hydrochloric acid methanol solution is added, and the absorbance is measured at 543 nm. The proposed method is simple, rapid, and useful for the determination of small amounts of the ketamine・HCl or quinine・HCl. No interference was observed with Ca^<2+>, Mg^<2+>, Fe^<3+>, Ni^<2+>, Cu^<2+>, Co^<2+>, Al^<8+>, Zn^<2+>, NO_3^-, Cl^-, glucose and lactose for the determination of ketamine・HCl or quinine・HCl.
公益社団法人日本薬学会の論文
1982-10-30

イオン会合性試薬による塩酸ケタミンおよび塩酸キニーネの吸光光度定量

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