ヒト血液あるいは尿中の微量クロルプロマジンの抽出

概要

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The chlorpromazine in the human blood or urine was determined by GC/MS. Trace amounts of chlorpromazine in the human blood or urine could not be completely extracted with organic solvent. However, the extraction efficiency was about 98-102% when aqueous solution saturated with tri-n-propylamine was added to the blood or urine. The recommended procedures are as follows : Place 20 ml blood or urine containing less than 1000 ng of chlorpromazine, 15% sodium carbonate aqueous solution, 1 ml of aqueous solution saturated with tri-n-propylamine, and 10 ml of benzene in 50 ml separatory funnel. Shake it for 2 min and centrifuge for 2 min at 3500 rpm in a 50 ml centrifuge tube. Place 5 ml of the benzene phase, and 1 ml of n-tricosane benzene solution (573 ng/ml) as an internal standard. Concentrate benzene on a water bath to 0.3 ml at 80±5℃. Determine the chlorpromazine by GC/MS. The extraction efficiency was 98% when as little as 10 ng of chlorpromazine・HCl was tested. When the amount of chlorpromazine in the human blood or urine was less than 28.8 μg, the extraction efficiency of chlorpromazine was higher in the presence of tri-n-propylamine compared to that without the base.
公益社団法人日本薬学会の論文
1988-10-31