バルビツール酸系及びベンゾジアゼピン系催眠薬のp-メチルベンジル誘導体化によるHPLC分析

概要

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An improved method for extracting barbiturates and benzodiazepines was examined. These drugs were reacted with α-chloro-p-xylene to form p-methylbenzyl derivatives, which were extracted with n-hexane. The experimental procedures were as follows ; Add 3 ml of 3% sodium carbonate solution, 0.3 ml of α-chloro-p-xylene and 10 ml of acetone to 2 ml of blood sample in 30 ml Elenmyer's flask. Warm it on a water bath for 15 min keeping the original volume by adding acetone, and for 15 min without adding acetone at 80±3℃. After cooling, add 2 ml of n-hexane. Shake it for 1 min and place it into a 15 ml test tube. Centrifuge the content of the test tube for 2 min at 3000 rpm. Take 10 μl of the n-hexane layer, and analyze it by HPLC. For the extraction of barbital or nitrazepam from blood sample no interference could be observed with the compounds, such as chloropromazine hydrochloride, perazine maleate, prochloroperazine maleate, glycine, phenyl alanine, glucose, and urea.
1987-04-30