血液, 尿中の数種の薬毒物の分析法の改良及び血痕経過時間の推定に関する研究

概要

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A complex of methamphetamine was formed in urine (10 ml) upon the addition of carbon disulfide, copper ion, and ammonia. Methamphetamine (≥ 20 μg) in urine was detected based on the infrared spectrum of the complex extracted with benzene within 20 min. Paraquat in blood (1 ml) or urine (2 ml) was adsorbed on silica gel or a florisil minicolumn. The paraquat (≥ 0.36 μg) was detected by passing a color-producing reagent through a minicolumn. The paraquat in the eluate was determined by colorimetry within 20 min. A simultaneous analysis of paraquat and diquat (≥ 0.5μg) in a phenolic extract of blood (1 ml) was achieved by second-derivative spectroscopy within 30 min. A gas-phase mini-circulation apparatus was made to recover hydrogen cyanide from blood (1 ml); the hydrogen cyanide (≥ 0.1μg) was determined by a modified pyridine-pyrazolone method. An overall examination was achieved within 45 min. Methemoglobin and carboxyhemoglobin in blood (3 μl) were determined simultaneously by absorbance at 563 nm within 10 min. These two methods were applied to blood taken from 70 victims of fire and auto exhausts for investigating the causes of death. The bloodstain (20 μl) age could be estimated by using an oxygen electrode and the total hemoglobin within 5 min.
社団法人日本分析化学会の論文
1999-05-05