酢酸鉛投与ラットの肝切片におけるカルシウム流出におよぼすカルチトニンの影響について

概要

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This experiment was made to examine whether the inhibitory effect of thyrocalcitonin (TCT) on calcium efflux is exhibited in liver slices from the lead-treated rats. Lead acetate solution of 20 mg Pb/100 g body weight was intraperitoneally administered. After 36 hr, animals were killed by decapitation. The liver slices were preincubated in 2 ml of krebs-Ringer bicarbonate buffer (pH 7.4) containing 2.5 μCi ^<45>Ca with 95% O_2-5% CO_2 as the gas phase. After preincubation, the slices were transferred to unlabeled media with or without TCT (8.0 MRC mU/ml), and incubated for an additional 30 or 60 min. Radioactivity was counted on a scintillation spectrometer. In the liver slices from normal ral, the rate of calcium efflux is inhibited in the presence of TCT, and the decrease of calcium efflux rate after TCT treatment was about 25% of the control. On the other hand, in the liver slices from the lead-treated rat, the calcium efflux rate was inhibited by TCT treatment. Approximately 30 or 50% of control decreased during the incubation period of 30 or 60 min, respectively. The present results suggest that liver calcium accumulation induced by lead administration is caused by the inhibitory effect of TCT on the calcium efflux in liver cells.
公益社団法人日本薬学会の論文
1975-10-31