Spectrophotometric Determination of Cephalexin and Ampicillin using o-Hydroxyhydroquinonephthalein and Palladium (II)

概要

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A new sensitive, rapid and simple spectrophotometric method for the determination of cephalexin (CEX) and ampicillin (AB-PC) was established using o-hydroxyhydroquinonephthalein (Qn. Ph.) and palladium (II) [Pd (II)] at a low concentration of cetyltrimethylammonium chloride (CTAC) in weak acidic media. This method is based on the fact that the intensity of the absorption peak of Qn. Ph. -Pd (II) complex at 630 nm is decreased significantly by the addition of CEX or AB-PC, and the decrease in the absorbance is proportional to the concentration of CEX or AB-PC. The method could be used in the concentration range up to -17μg/10 ml of CEX and -14μg/10 ml of AB-PC, where the spectrophotometric sensitivities were estimated to be 0.0015μg/cm^2 for CEX and 0.0012μg/cm^2 for AB-PC for an absorbance unit of 0.001 at 630 nm, and was applied to the determination of CEX or AB-PC in pharmaceutical preparations.
公益社団法人日本薬学会の論文
1982-07-25