o-ヒドロキシヒドロキノンフタレインとウラン(VI)を用いるエチル炭酸キニーネの吸光光度定量

概要

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A simple and rapid Spectrophotometric method for the determination of quinine ethylcarbonate (QNEC) with o-hydroxyhydroquinonephthalein (Qn.Ph.) and uranium(VI) {U(VI)} has been established. This method based on the formation of association complex of Qn.Ph.-U(VI)-QNEC can be applied in the concentration range of 0〜120 *g/10 ml of QNEC. The Sandell sensitivity is estimated to be 0.0098 *g/cm^2 for QNEC at an absorbance of 0.001 at 555 nm against Qn.Ph.-U(VI) solution, and the apparent molar absorptivity is 4.1 × 10^4. The recommended analytical procedure is as follows : QNEC less than 120 *g was placed in a 10-ml measuring flask, and 2.0 ml of 0.5 % MC solution, 0.75ml of 1.0 × 10^<-3>M U(VI) solution, 2.0 ml of a Walpole buffer (sodium acetateacetic acid, pH 5.3) solution and 1.5ml of 1.0 × 10^<-3>M Qn.Ph. solution were added. Then the mixture was diluted to 10.0 ml with water, and was kept at (20〜25) ℃ for 20 min. The difference of absorbance between the Qn.Ph.-U(VI)-QNEC solution and Qn.Ph.-U(VI) solution was measured at 555 nm against water. The mole ratio of U(VI), Qn.Ph. and QNEC in the association complex was estimated to be 1 : 2 : 2 by the molar ratio method. The proposed method was applied to the determination of QNEC in pharmaceutical preparations.
社団法人日本分析化学会の論文
1982-08-05