クロロガレインメチルエステルを用いる長鎖第4級アンモニウム塩の吸光光度定量

概要

論文の詳細を見る
A highly sensitive and simple spectrophotometry for a small amount of long chain quaternary ammonium salts (R_4NX) was developed by using chlorogallein methylester (Cl. Gall. CH_3) as a reagent. Maximum difference of absorbance between Cl. Gall. CH_3 solution and Cl. Gall. CH_3-R_4NX solution is observed at 600 nm, and the absorbance is constant in the pH range from 4.3 to 5.4. The calibration curve was linear in the range of 5 to 20 μg/10 ml cetylpyridinium (CP), cetyltrimethylammonium (CTA) and benzethonium (Benz.). This method was applicable to the analyses of cationic surface-active agent in waste water. The recommended analytical procedure for R_4NX salts is as follows. A sample solution containing less than 20 μg of R_4NX salts (CP, CTA, Benz.) is taken in a 10 ml measuring flask, and then 2.0 ml of Walpole buffer solution (pH 4.6), 1.0 ml of 1.0% polyvinylalcohol (PVA) solution and 1.0 ml of 1.0×10^<-3>M Cl. Gall. CH_3 methanol solution are added, and diluted to the mark with distilled water, and the solution is kept at (20〜25) ℃ for 30 min, and then the difference of absorbance of Cl. Gall. CH_3 solution and Cl. Gall. CH_3-R_4NX solution is measured at 600 nm. Bismuth (III), thorium(IV), zirconium(IV) and iron(III) could be masked by the addition of EDTA or CyDTA solution, and aluminum(III) and tin(IV) could be masked by sodium fluoride or sodium oxalate solution.
社団法人日本分析化学会の論文
1979-05-05