ピロガロールレッド-モリブデン(VI)錯体を用いる硫酸キニーネの定量

概要

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A rapid and simple method for the spectrophotometric determination of micro amounts of quinine sulfate with pyrogallol red(PR) and molybdenum(VI) in the presence of polyoxyethylenesorbitanmonolaurate (LT-221) at pH4.5 was established. The calibration curve for the spectrophotometric determination was linear in the range of 0〜1.12mg quinine sulfate/10ml. The sensitivity was 0.082μg quinine sulfate/ml for absorbance of 0.001. The recommended analytical procedure is as follows. A sample solution containing less than 1.12mg of quinine sulfate is taken in a 10ml measuring flask, and 1.0ml of 5.0×lO^<-4>M molybdenum(VI), 0.5ml of 2.0% LT-221 solution and 0.5ml of 1.0×10^<-3>M PR solution are added, and then the resulting solution is adjusted to pH4.5 with Walpole acetate buffer solution. The content is diluted to the mark with water, kept at (20〜25)℃ for 50min, and the absorbance of the PR-molybdenum (VI)-quinine sulfate solution (A solution) is measured at 610nm against PR-molybdenum(VI) solution (B solution). The effect of foreign substances on the absorbance were examined. Aluminum(III), iron(III) and chromium (VI) interfered, but could be masked by the addition of sodium fluoride or EDTA solution.
1981-10-05