2,4,5,7-テトラクロロフルオレセインとパラジウム(II)による塩酸パパベリンの吸光光度定量

概要

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A color reaction of papaverine hydrochloride (Papav. HCl) utilizing a ternary-complex system among Papav., a metal ion, and a halogeno-fluorescein was studied, and fundamental conditions for the spectrophotometric method of Papav. HCl with 2, 4, 5, 7-tetrachlorofluorescein (T. Cl. fl) and palladium (II) {Pd (II)} were discussed. The standard procedure obtained is as follows ; Up to 75μg of Papav. HCl is placed in a 10-ml measuring flask with 0.75 ml of 1.0% polyvinyl alcohol (PVA) solution, 1.0ml of 1.0×10^<-3>M Pd (II) solution, 2.5 ml of Walpole buffer solution (sodium acetateacetic acid, pH 5.2) and 1.0 ml of 1.0×10^<-3> M T. Cl. fl solution. The solution is diluted to 10 ml with water, kept at 40℃ for 15 min and cooled in water for 5 min. Then the absorption difference between the T. Cl. fl-Pd(II)-Papav. and T. Cl. fl-Pd(II) solutions is measured at 540 nm. The apparent molar absorptivity was 4.0×10^4 dm^3 mol^<-1> cm^<-1>. The molar ratio of [Pd (II) : T. Cl. fl : Papav.] in this color reaction was estimated to be 1 : 1 : 1 by continuous variation method and mole ratio methods. The proposed method was applied to the determination of Papav. HCl in pharmaceutical preparations and the analytical results were satisfactory.
社団法人日本分析化学会の論文
1985-10-05