Compositional Analysis of Hyaluronan, Chondroitin Sulfate and Dermatan Sulfate : HPLC of Disaccharides Produced from the Glycosaminoglycans by Solvolysis
スポンサーリンク
概要
- 論文の詳細を見る
An ion-exchange high-performance liquid chromatography procedure was developed for analysis of mixtures of N-acetyldermosine, N-acetylchondrosine and N-acetylhyalobiuronic acid produced quantitatively by heating dermatan sulfate, chondroitin sulfate and hyaluronan in dimethyl sulfoxide (DMSO) containing 0.1% (v/v) H_2O at 80℃ for 48 h. These disaccharides were eluted from a TSK gel SAX column using 0.1 M acetic acid containing KCl, and detected fluorometrically by post column derivatization. The eluate was mixed with 2-cyanoacetamide solution and alkaline solution, and heated at 110℃ for 4 min. The resultant compounds were detected fluorometrically (Ex. 335 nm and Em. 390 nm). The usefulness and practicality of the present method were verified by applications to the determination of glycosaminoglycans in tissues.
- 公益社団法人日本薬学会の論文
- 1996-05-15
著者
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IMANARI Toshio
Faculty of Pharmaceutical Sciences, Chiba University
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Toida Toshihiko
Graduate School of Pharmaceutical Sciences, Chiba University
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Takayama Hiromitsu
Laboratory Of Molecular Structure And Biological Function Graduate School Of Pharmaceutical Sciences
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Toida T
Department Ofbioanalytical Chemistry Graduate School Of Pharmaceutical Sciences Chiba University
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Toida Toshihiko
Faculty Of Pharmaceutical Sciences Chiba University
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Imanari^ Toshio
Faculty Of Pharmaceutical Sciences Chiba University
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Imanari Toshio
Faculty Of Pharmaceutical Sciences Chiba University
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Toyoda Hidenao
Faculty Of Pharmaceutical Sciences Chiba University
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Koshiishi I
Faculty Of Pharmaceutical Sciences Chiba University
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Koshiishi Ichiro
Faculty Of Pharmaceutical Sciences Chiba University
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Ikei Tatsuo
Faculty Of Pharmaceutical Sciences Chiba University
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QIU Guoning
Faculty of Pharmaceutical Sciences, Chiba University
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Imanari T
Faculty Of Pharmaceutical Sciences Chiba University
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Qiu Guoning
Faculty Of Pharmaceutical Sciences Chiba University
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