Fundamental Studies on Clinical Chemistry.XI.A New Micromethod for the Determination of General Ketosteroids using p-Nitrophenylhydrazine Reagent
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概要
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A highly sensitive spectrophotometric method for the determination of general ketosteroids was investigated. The development of chromophore with p-nitrophenylhydrazine reagent was achieved efficiently in alkaline dimethylformamide. Micro amounts (0〜50 μg. for saturated ketosteroids ; 0〜25 μg. for Δ^4-3-ones) of steroids were determined by the standard procedure. One of the remarkable advantages is the possibility to estimate micro quantities of the saturated 3-ketosteroids such as cholestan-3-one, that has been almost impossible by the most published methods. A discussion on the reactivities of different kinds of steroidal keto group for the method derived was also presented from the results of the study using forty oxosteroids.
- 公益社団法人日本薬学会の論文
- 1967-04-25
著者
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木村 道也
Faculty of Pharmaceutical Sciences, Hokkaido University
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仁科 甫啓
Faculty Of Pharmaceutical Sciences University Of Hokkaido
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木村 道也
Faculty Of Pharmaceutical Sciences Hokkaido University
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坂本 嵩
Faculty of Pharmaceutical Sciences, University of Hokkaido
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坂本 嵩
Faculty Of Pharmaceutical Sciences University Of Hokkaido
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