CHANGES OF PEROXISOMES IN THE REGENERATING HEPATIC PARENCHYMAL CELLS IN THE RAT

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Changes of peroxisomes in the rat hepatic parenchymal cells during the regeneration following partial hepatectomy were investigated ultracytochemically. For the demonstration of peroxidatic activity of catalase frozen and nonfrozen sections from tissues fixed in 2% glutaraldehyde for 2 hours at 0℃ or 20℃ were incubated in Novikoff-Goldfischer medium (1969) mainly at pH 9.0 for 1 to 2 hours at 37℃. After incubation, they were post-osmificated and processed for electron microscopic observation. The peroxidase activity was demonstrated in peroxisomes (0.2-0.7 μ) and occasionally in outer and intracristal spaces of mitochondria in the normal and regenerating hepatic parenchymal cells. The smaller peroxisomes (0.1-0.2 μ) having weaker activity may be the developing peroxisomes. In Kupffer cells, cisternae of the endoplasmic reticulum showed the positive enzymatic activity. The number of peroxisomes per cell calculated on the toluidine blue-stained epon sections (1 μ thick) showed decrease till 24 hours following hepatectomy. However, the number of peroxisomes revealed gradual recovery at 28-30 hours. Marked decrease of the peroxisome number in hepatic parenchymal cells was noticed in the normal rat, which received a single intraperitoneal injection of Actinomycin D (25 μg./100 g body weight).
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