ULTRACYTOCHEMICAL STUDIES OF ADENOSINE NUCLEOTIDASES IN AORTIC ENDOTHELIAL AND SMOOTH MUSCLE CELLS : Ca^<++>-ATPase AND Na^+,K^+-ATPase
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概要
- 論文の詳細を見る
Ca^<++>-activated adenosine triphosphatase (Ca^<++>-ATPase) and ouabainsensitive, K^+-dependent p-nitrophenylphosphatase (K^+-NPPase), a component of Na^+,K^+-ATPase system, were studied in rat aortic endothelium and smooth muscle cell using the recent improved cytochemical methods introduced by Ando et al. (1981) and Mayahara et at. (1980) respectively. In the endothelium, Ca^<++>-ATPase activity was localized on the external side of luminal, lateral and abluminal membrane, on the inside of caveolae and vesicles of both luminal and abluminal surfaces and on the matrix of mitochondria. Furthermore, Ca^<++>-ATPase activity on only the luminal surface was affected by the concentrations of Ca^<++> in reaction medium. K^+-NPPase activity was recognized on the cytoplasmic side of or exactly on the membrane covering abluminal caveolae, abluminal vesicles and Golgi apparatus. These findings indicate the existence of a cytochemical difference on the aortic endothelial cell-surface corresponding to the function. On the other hand, in the smooth muscle cell, reaction products indicating Ca^<++>-ATPase activity were localized on the plasma membrane, the caveolae, the sarcoplasmic reticulum, the Golgi apparatus and the matrix of mitochondria. K^+-NPPase activity was observed on the cytoplasmic side of or just on the caveolae and the Golgi apparatus in the smooth muscle cell. These findings confirm the previously reported biochemical studies and support the mechanism of the smooth muscle relaxation in relation to the sequestration or extrusion of Ca^<++> and the function of adenine nucleotides and nucleosides.
- 日本組織細胞化学会の論文
著者
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Fujimoto K
Department Of Biochemistry Meikai University School Of Dentistry
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Fujimoto Kazushi
Department Of Anatomy And Neurobiology Graduate School Of Medicine Kyoto University
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Ogawa Kazushige
Department Of Veterinary Anatomy Graduate School Of Agricultural And Biological Sciences Osaka Prefe
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Ogawa Kazuo
Department Of Anatomy Faculty Of Medicine Kyoto University
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Fujimoto Kazushi
Section Of Physiological Anatomy Fukui Prefectural University Faculty Of Nursing And Welfare Laborat
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Ogawa Kazuo
Department Of Anatany Faculty Of Medicine Kyoto University
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