高速液体クロマトグラフィー/質量分析法による大腸菌ホスファチジルグリセロールの分子種分析

概要

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A simple and sensitive method for the determination of molecular species of bacterial phosphatidylglycerols (1, 2-diacyl-sn-glycero-3-phospho-1′-sn-glycerol, PG) as an intact form is described. The PG fraction isolated from Escherichia coli IAM12119 was subjected to reversed phase high-performance liquid chromatography (HPLC) in conjunction with ion-trap mass spectrometry with electrospray ionization (ESI-MS). The HPLC analysis was performed with an ODS column (25 cm×4.6 mm i.d., 5 μm particle size) using methanol/water/aqueous ammonium hydroxide (93 : 7 : 0.1, v/v/v) as the mobile phase, which gave a clear resolution of individual molecular species containing saturated, monounsaturated and cyclopropane fatty acids. In addition to a prominent deprotonated [M-H]^- molecule, the negative ESI-MS gave weak product ions, [M-RCO]^-, [M-H-RCOOH]^- and [RCOO]^-, by which individual molecular species could be identified. These product ions, derived from a fatty acid at the sn-2 position, showed greater intensities than those from the sn-1 position.
社団法人日本分析化学会の論文
2005-02-05