ヒドロキサムセファデックスの作製とその性質

概要

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A new chelating ion exchanger named "hydroxamized Sephadex" (H-Sephadex) was prepared and its properties were investigated. H-Sephadex was prepared by the reaction of hydroxylamine with carboxy methyl-Sephadex (4.27 mmol COOH/g-gel,Pharmacia). The content of the introduced hydrox-amic acid residue was 0.87 mmol/g-gel. Lead(II), copper(II), mercury(II), cadmium(II), zinc(II),and manganese(II) were collected completely from 50 ml of 1 × 10^<-5>M solution of each metal salt over the pH range of 3.2〜5.4, 4.0〜9.6, 4.2〜8.4, 4.7〜9.8, 4.8〜9.6, and 5.1〜9.6, respectively, by stirring with 0.1 g of H-Sephadex for 30 min at room temperature. Copper was collected completely from the solutions containing 1 M sodium nitrate or calcium nitrate at pH 7.6〜8.7, but the collection of cadmium and mercury was incomplete under similar conditions. When the collection of copper was carried out from 1 M sodium chloride, the optimum pH range was shifted to 9.0〜9.6. The collection of copper, cadmium, and mercury was complete from 10^<-4>M sodium citrate but incomplete from 10^<-6>M EDTA at pH 5.0〜6.9. With 0.1 g of H-Sephadex, copper, cadmium, and mercury were completely collected from (10〜1000) ml solutions containing 5 × 10^<-4>mmol of the metal salt at pH 5.2〜6.9 by stirring for (30〜60) min at room temperature.
社団法人日本分析化学会の論文
1982-08-05