セミミクロカラムとカラムスイッチング法を用いる高速液体クロマトグラフィーによる血清中プロポフォールの定量

概要

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An analytical method for the determination of propofol (2,6-diisopropylphenol) in human serum was investigated by high-performance liquid chromatography using a semimicro bore column and a column-switching technique. The semi-micro bore column {TSKgel ODS-80Ts (2mm i.d.×150mm)} gave a higher peak height (ca. 3.5 folds) compared with that of a conventional column (4.6 mm i.d.). As the result of an examination of some pretreatment columns, higher recoveries of propofol on TSK precolumn BSA-ODS and TSKprecolumn PW were obtained. TSKprecolumn PW was adopted as a pretreatment column, because its less adsorptive ability was considered to be effective in eliminating the hydrophobic compounds in human serum. Under the optimized analytical conditions, the linearity of the calibration curve was obtained over the range of 5〜300ng/ml and the detection limit was 1.5ng/ml (S/N=3). The relative standard deviation against a practical sample was 2.4% (n=6). As a consequence, this method was suggested to be utilized for a highly sensitive quantitative analysis of propofol in human serum of an anesthetized patient.
社団法人日本分析化学会の論文
1997-04-05