高速液体クロマトグラフィーによる血清中ヨヒンビンの定量

概要

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An automated method for determination of yohimbine (Yoh) in the serum was developed by means of column switching high performance liquid chromatography (HPLC). TSK-precolumn BSA-ODS and TSK-gel ODS-120T were used as a precolumn and analytical column, respectively. The wavelengths of detection were used at 280 nm (excitation) and 360 nm (emission). A 100 μl serum sample is directly injected onto the precolumn. Yoh is then eluted within 30 min with an methanol-potassium phosphate buffer mixture. The analytical recoveries (99.3-110.4%), reproducibilities (within-run, C.V.<2.27%), and detection limit (0.80 ng/ml, S/N=3) indicate that this system is suited for determination of Yoh. The five healthy volunteers received a single oral dose 10 mg of HYoh powder. The average of the maximal serum concentration and the area under the curve (AUC) from 0 to 4 h were 10.3±0.88 ng/ml and 19.70±0.87 ng・h・ml^<-1>, respectively. The elimination rate constant (K_<01>) was 0.52±0.03 h, and biological half-life (t_<1/2el>) was 1.32±0.12 h.
社団法人日本薬学会の論文
1990-04-25